{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T23:33:36Z","timestamp":1784590416449,"version":"3.55.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R01 AI148437"],"award-info":[{"award-number":["R01 AI148437"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2024,7,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Candida auris<\/jats:italic>\n              is a multidrug-resistant fungal pathogen with a propensity to colonize humans and persist on environmental surfaces.\n              <jats:italic>C. auris<\/jats:italic>\n              invasive fungal disease is being increasingly identified in acute and long-term care settings. We have developed a prototype cartridge-based\n              <jats:italic>C. auris<\/jats:italic>\n              surveillance assay (CaurisSurV cartridge; \u201cresearch use only\u201d) that includes integrated sample processing and nucleic acid amplification to detect\n              <jats:italic>C. auris<\/jats:italic>\n              from surveillance skin swabs in the GeneXpert instrument and is designed for point-of-care use. The assay limit of detection (LoD) in the skin swab matrix was 10.5 and 14.8 CFU\/mL for non-aggregative (AR0388) and aggregative (AR0382) strains of\n              <jats:italic>C. auris<\/jats:italic>\n              , respectively. All five known clades of\n              <jats:italic>C. auris<\/jats:italic>\n              were detected at 2-3-5\u00d7 (31.5\u201352.5 CFU\/mL) the LoD. The assay was validated using a total of 85 clinical swab samples banked at two different institutions (University of California Los Angeles, CA and Wadsworth Center, NY). Compared to culture, sensitivity was 96.8% (30\/31) and 100% (10\/10) in the UCLA and Wadsworth cohorts, respectively, providing a combined sensitivity of 97.5% (40\/41), and compared to PCR, the combined sensitivity was 92% (46\/50). Specificity was 100% with both clinical (\n              <jats:italic>C. auris<\/jats:italic>\n              negative matrix,\n              <jats:italic>N<\/jats:italic>\n              = 31) and analytical (non-\n              <jats:italic>C<\/jats:italic>\n              .\n              <jats:italic>auris<\/jats:italic>\n              strains,\n              <jats:italic>N<\/jats:italic>\n              = 32) samples. An additional blinded study with\n              <jats:italic>N<\/jats:italic>\n              = 60 samples from Wadsworth Center, NY yielded 97% (29\/30) sensitivity and 100% (28\/28) specificity. We have developed a completely integrated, sensitive, specific, and 58-min prototype test, which can be used for routine surveillance of\n              <jats:italic>C. auris<\/jats:italic>\n              and might help prevent colonization and outbreaks in acute and chronic healthcare settings.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              This study has the potential to offer a better solution to healthcare providers at hospitals and long-term care facilities in their ongoing efforts for effective and timely control of\n              <jats:italic>Candida auris<\/jats:italic>\n              infection and hence quicker response for any potential future outbreaks.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/jcm.00525-24","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T13:01:09Z","timestamp":1718715669000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["A simple and sensitive test for\n            <i>Candida auris<\/i>\n            colonization, surveillance, and infection control suitable for near patient use"],"prefix":"10.1128","volume":"62","author":[{"given":"Sukalyani","family":"Banik","sequence":"first","affiliation":[{"name":"Center for Emerging Pathogens, Department of Medicine, Public Health Research Institute, Rutgers New Jersey Medical School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burcu","family":"Ozay","sequence":"additional","affiliation":[{"name":"Research and Development, Cepheid, Sunnyvale, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisol","family":"Trejo","sequence":"additional","affiliation":[{"name":"UCLA DGSOM Pathology &amp; Lab Medicine, UCLA, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YanChun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Mycology laboratory, Wadsworth Center, Albany, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charan","family":"Kanna","sequence":"additional","affiliation":[{"name":"Center for Emerging Pathogens, Department of Medicine, Public Health Research Institute, Rutgers New Jersey Medical School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Santellan","sequence":"additional","affiliation":[{"name":"UCLA DGSOM Pathology &amp; Lab Medicine, UCLA, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bennett","family":"Shaw","sequence":"additional","affiliation":[{"name":"UCLA DGSOM Pathology &amp; Lab Medicine, UCLA, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6232-5535","authenticated-orcid":true,"given":"Sukantha","family":"Chandrasekaran","sequence":"additional","affiliation":[{"name":"UCLA DGSOM Pathology &amp; Lab Medicine, UCLA, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0906-8426","authenticated-orcid":true,"given":"Sudha","family":"Chaturvedi","sequence":"additional","affiliation":[{"name":"Mycology laboratory, Wadsworth Center, Albany, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindy","family":"Vejar","sequence":"additional","affiliation":[{"name":"Research and Development, Cepheid, Sunnyvale, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soumitesh","family":"Chakravorty","sequence":"additional","affiliation":[{"name":"Center for Emerging Pathogens, Department of Medicine, Public Health Research Institute, Rutgers New Jersey Medical School, Newark, New Jersey, USA"},{"name":"Research and Development, Cepheid, Sunnyvale, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5196-1571","authenticated-orcid":true,"given":"David","family":"Alland","sequence":"additional","affiliation":[{"name":"Center for Emerging Pathogens, Department of Medicine, Public Health Research Institute, Rutgers New Jersey Medical School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5217-6142","authenticated-orcid":true,"given":"Padmapriya","family":"Banada","sequence":"additional","affiliation":[{"name":"Center for Emerging Pathogens, Department of Medicine, Public Health Research Institute, Rutgers New Jersey Medical School, Newark, New Jersey, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.7326\/M22-3469"},{"key":"e_1_3_4_3_2","unstructured":"CDC. 2023. 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