{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:28:51Z","timestamp":1762176531693},"reference-count":31,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Fast, reliable, and versatile typing tools are essential to differentiate among related bacterial strains for epidemiological investigation and surveillance of health care-associated infection with multidrug-resistant (MDR) pathogens. The DiversiLab (DL) system is a semiautomated repetitive-sequence-based PCR system designed for rapid genotyping. The DL system performance was assessed by comparing its reproducibility, typeability, discriminatory power, and concordance with those of pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) and by assessing its epidemiological concordance on well-characterized MDR bacterial strains (\n            <jats:italic>n<\/jats:italic>\n            = 165). These included\n            <jats:italic>vanA Enterococcus faecium<\/jats:italic>\n            , extended-spectrum \u03b2-lactamase (ESBL)-producing strains of\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Acinetobacter baumannii<\/jats:named-content>\n            , and ESBL- or metallo-\u03b2-lactamase (MBL)-producing\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>\n            strains. The DL system showed very good performance for\n            <jats:named-content content-type=\"genus-species\">E. faecium<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">K. pneumoniae<\/jats:named-content>\n            and good performance for other species, except for a discrimination index of &lt;95% for\n            <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            (93.9% and 93.5%, respectively) and incomplete concordance with MLST for\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            (78.6%) and\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            (97.0%). Occasional violations of MLST assignment by DL types were noted for\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            . Complete epidemiological concordance was observed for all pathogens, as all outbreak-associated strains clustered in identical DL types that were distinct from those of unrelated strains. In conclusion, the DL system showed good to excellent performance, making it a reliable typing tool for investigation of outbreaks caused by study pathogens, even though it was generally less discriminating than PFGE analysis. For\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            , MLST cannot be reliably inferred from DL type due to phylogenetic group violation or discordance.\n          <\/jats:p>","DOI":"10.1128\/jcm.00528-11","type":"journal-article","created":{"date-parts":[[2011,8,4]],"date-time":"2011-08-04T14:08:28Z","timestamp":1312466908000},"page":"3616-3620","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Controlled Performance Evaluation of the DiversiLab Repetitive-Sequence-Based Genotyping System for Typing Multidrug-Resistant Health Care-Associated Bacterial Pathogens"],"prefix":"10.1128","volume":"49","author":[{"given":"Ariane","family":"Deplano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Denis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hector","family":"Rodriguez-Villalobos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raf","family":"De Ryck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc J.","family":"Struelens","sequence":"additional","affiliation":[{"name":"Present address: Scientific Advice Unit, European Centre for Disease Prevention and Control, Stockholm, Sweden."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Hallin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"48th Intersci. 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