{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T23:33:08Z","timestamp":1790033588165,"version":"4.0.1"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A fundamental role for the endosymbiotic bacteria\n            <jats:named-content content-type=\"genus-species\">Wolbachia pipientis<\/jats:named-content>\n            in the pathogenesis of\n            <jats:named-content content-type=\"genus-species\">Dirofilaria immitis<\/jats:named-content>\n            infections has emerged in recent years. Diagnostic opportunities arising from this breakthrough have not yet been fully exploited. This study was aimed at developing conventional and real-time PCR assays to carry out a molecular survey in a convenience sample of cats living in an area where\n            <jats:named-content content-type=\"genus-species\">D. immitis<\/jats:named-content>\n            is endemic and to evaluate the detection of bacterial DNA in blood as a surrogate assay for diagnosing filaria-associated syndromes in cats. COI and FtsZ loci were used as targets for\n            <jats:named-content content-type=\"genus-species\">D. immitis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>\n            PCR assays, respectively, and real-time TaqMan PCR assays were used only for\n            <jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>\n            . A convenience sample of 307 disease-affected or healthy cats examined at a University facility were PCR tested, and their medical records were investigated. Conventional nested PCR for\n            <jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>\n            amplified the endosymbionts of both\n            <jats:named-content content-type=\"genus-species\">D. immitis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">D. repens<\/jats:named-content>\n            , while real-time PCR was highly specific only for the former. Observed prevalences of 0.3 and 10.4% were found using conventional nested PCR assays for\n            <jats:named-content content-type=\"genus-species\">D. immitis<\/jats:named-content>\n            and real-time PCR for\n            <jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>\n            , respectively. Similar prevalences were established using the Wolbachia nested PCR (98% concordance with real-time PCR). The group of\n            <jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>\n            -positive samples had a significantly higher proportion of subjects with respiratory signs (29.0% versus 9.7%;\n            <jats:italic>P<\/jats:italic>\n            = 0.002). The findings of this study indicate that a highly sensitive PCR assay can be used to detect the\n            <jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>\n            organism in the peripheral blood of cats with respiratory signs.\n          <\/jats:p>","DOI":"10.1128\/jcm.00528-12","type":"journal-article","created":{"date-parts":[[2012,5,31]],"date-time":"2012-05-31T05:34:45Z","timestamp":1338442485000},"page":"2624-2630","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Detection of Wolbachia DNA in Blood for Diagnosing Filaria-Associated Syndromes in Cats"],"prefix":"10.1128","volume":"50","author":[{"given":"Maria Elena","family":"Turba","sequence":"first","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Zambon","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augusta","family":"Zannoni","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samanta","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio","family":"Gentilini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1996.0022"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.5326\/0400376"},{"key":"e_1_3_1_4_2","first-page":"7","article-title":"Feline heartworm disease: solving the puzzle","volume":"2007","author":"Blagburn BL","year":"2007","unstructured":"BlagburnBL DillonAR. 2007. 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