{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:46:42Z","timestamp":1773287202824,"version":"3.50.1"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The severe morbidity of human brucellosis is one of the main reasons for using molecular typing in the epidemiological surveillance of this worldwide zoonosis. Multiple-locus variable-number repeat analysis (MLVA-16), hypervariable octameric oligonucleotide fingerprinting (HOOF-print), and the differences in the single nucleotide polymorphisms (SNPs) (codons 1249 and 1309) of the DNA-dependent RNA polymerase \u03b2 subunit (<jats:italic>rpoB<\/jats:italic>) were used to type a human<jats:italic>Brucella melitensis<\/jats:italic>population (108 strains) collected from throughout Spain over 13 years. Eighty-six MLVA types (discriminatory index, 0.99) were detected, with a wide-ranging genetic similarity coefficient (37.2 to 93.7%). The population clustered into the following groups: American, with genotypes 47 (1 strain), 48 (13 strains), 53 (12 strains), 55 (2 strains), 80 (1 strain), and a new genotype (2 strains), Western Mediterranean, with genotype 51 (9 strains), and Eastern Mediterranean, with genotypes 42 (60 strains), 43 (4 strains), and 63 (4 strains). Two profession-related and two foodborne acquisitions were confirmed. Distributed throughout Spain, Eastern Mediterranean genotype 42 was the most common (55%). The low MLVA-16 allelic polymorphism (genetic similarity range, 75 to 94%) of the genotype 42 strains suggests that they recently evolved from a common ancestor.<jats:italic>rpoB<\/jats:italic>typing grouped the strains as<jats:italic>rpoB<\/jats:italic>type 1 (1249-ATG\/1309-CTG; 28.7%),<jats:italic>rpoB<\/jats:italic>type 2 (1249-ATG\/1309-CTA; 62.9%), and<jats:italic>rpoB<\/jats:italic>type 3 (1249-ATA\/1309-CTG; 8.3%). According to the MLVA-16 results, the population clustered by<jats:italic>rpoB<\/jats:italic>type. Given the correlation between<jats:italic>B. melitensis<\/jats:italic>MLVA groups and<jats:italic>rpoB<\/jats:italic>types (American and<jats:italic>rpoB<\/jats:italic>type 1, Eastern Mediterranean and<jats:italic>rpoB<\/jats:italic>type 2, and Western Mediterranean and<jats:italic>rpoB<\/jats:italic>type 3), the<jats:italic>rpoB<\/jats:italic>type could be used as an initial marker for the epidemiological surveillance of brucellosis.<\/jats:p>","DOI":"10.1128\/jcm.00533-10","type":"journal-article","created":{"date-parts":[[2010,6,17]],"date-time":"2010-06-17T01:56:56Z","timestamp":1276739816000},"page":"2734-2740","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Epidemiological and Phylogenetic Analysis of Spanish Human<i>Brucella melitensis<\/i>Strains by Multiple-Locus Variable-Number Tandem-Repeat Typing, Hypervariable Octameric Oligonucleotide Fingerprinting, and<i>rpoB<\/i>Typing"],"prefix":"10.1128","volume":"48","author":[{"given":"Sylvia","family":"Valdezate","sequence":"first","affiliation":[{"name":"Servicio de Bacteriologi\u0301a, Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Navarro","sequence":"additional","affiliation":[{"name":"Servicio de Bacteriologi\u0301a, Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilar","family":"Villalo\u0301n","sequence":"additional","affiliation":[{"name":"Servicio de Bacteriologi\u0301a, Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gema","family":"Carrasco","sequence":"additional","affiliation":[{"name":"Servicio de Bacteriologi\u0301a, Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan A.","family":"Sae\u0301z-Nieto","sequence":"additional","affiliation":[{"name":"Servicio de Bacteriologi\u0301a, Centro Nacional de Microbiologi\u0301a, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.12.015"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2008.10.007"},{"key":"e_1_3_2_4_2","unstructured":"Techniques for the brucellosis laboratory. 1988"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.11.2660-2666.1994"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-3-15"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-37"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_3_2_9_2","first-page":"L\/204\/39","volume":"52","year":"2009","unstructured":"European Commission. 2009. 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