{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:33:48Z","timestamp":1761129228966},"reference-count":10,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A multitarget PCR was developed for the direct detection of penicillinase-producing\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.\n          <\/jats:p>","DOI":"10.1128\/jcm.00540-15","type":"journal-article","created":{"date-parts":[[2015,5,21]],"date-time":"2015-05-21T02:21:08Z","timestamp":1432174868000},"page":"2706-2708","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Multitarget PCR Assay for Direct Detection of Penicillinase-Producing Neisseria gonorrhoeae for Enhanced Surveillance of Gonococcal Antimicrobial Resistance"],"prefix":"10.1128","volume":"53","author":[{"given":"Cameron","family":"Buckley","sequence":"first","affiliation":[{"name":"Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia"},{"name":"The University of Queensland, Brisbane, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ella","family":"Trembizki","sequence":"additional","affiliation":[{"name":"Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia"},{"name":"The University of Queensland, Brisbane, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W.","family":"Baird","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcus","family":"Chen","sequence":"additional","affiliation":[{"name":"Melbourne Sexual Health Centre, Alfred Health, Carlton Victoria, Australia"},{"name":"Central Clinical School, Monash University, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basil","family":"Donovan","sequence":"additional","affiliation":[{"name":"Kirby Institute, University of New South Wales, Sydney, NSW, Australia"},{"name":"Sydney Sexual Health Centre, Sydney Hospital, Sydney, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Freeman","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Namraj","family":"Goire","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia"},{"name":"Nepean Clinical School, University of Sydney, Kingswood, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Guy","sequence":"additional","affiliation":[{"name":"Kirby Institute, University of New South Wales, Sydney, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica M.","family":"Lahra","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Regan","sequence":"additional","affiliation":[{"name":"Kirby Institute, University of New South Wales, Sydney, NSW, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Whiley","sequence":"additional","affiliation":[{"name":"Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia"},{"name":"The University of Queensland, Brisbane, Queensland, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Antibiotic resistance threats in the United States, 2013","author":"Centers for Disease Control and Prevention","year":"2013","unstructured":"Centers for Disease Control and Prevention. 2013. Antibiotic resistance threats in the United States, 2013. U.S. Department of Health and Human Services, Atlanta, GA. http:\/\/www.cdc.gov\/drugresistance\/threat-report-2013\/."},{"key":"e_1_3_2_3_2","volume-title":"Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae","author":"World Health Organization","year":"2012","unstructured":"World Health Organization. 2012. Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae. World Health Organization, Geneva, Switzerland. http:\/\/whqlibdoc.who.int\/publications\/2012\/9789241503501_eng.pdf."},{"key":"e_1_3_2_4_2","volume-title":"Cephalosporin-resistant Neisseria gonorrhoeae public health response plan","author":"Centers for Disease Control and Prevention","year":"2012","unstructured":"Centers for Disease Control and Prevention. 2012. Cephalosporin-resistant Neisseria gonorrhoeae public health response plan. Division of STD Prevention, National Center for HIV, STD, and TB Prevention, Atlanta, GA."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3217"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02024-10"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02993-14"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/45.6.777"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr162"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt501"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000154495.24519.bf"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00540-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00540-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:22:30Z","timestamp":1646482950000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00540-15"}},"subtitle":[],"editor":[{"given":"E.","family":"Munson","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,8]]},"references-count":10,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1128\/JCM.00540-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00540-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8]]},"assertion":[{"value":"2015-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}