{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:59:53Z","timestamp":1791356393641,"version":"4.3.1"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Worldwide, nontuberculous mycobacteria (NTM) have become emergent pathogens of pulmonary infections in cystic fibrosis (CF) patients, with an estimated prevalence ranging from 5 to 20%. This work investigated the presence of NTM in sputum samples of 129 CF patients (2 to 18 years old) submitted to longitudinal clinical supervision at a regional reference center in Rio de Janeiro, Brazil. From June 2009 to March 2012, 36 NTM isolates recovered from 10 (7.75%) out of 129 children were obtained. Molecular identification of NTM was performed by using PCR restriction analysis targeting the\n            <jats:italic>hsp65<\/jats:italic>\n            gene (PRA-\n            <jats:italic>hsp65<\/jats:italic>\n            ) and sequencing of the\n            <jats:italic>rpoB<\/jats:italic>\n            gene, and susceptibility tests were performed that followed Clinical and Laboratory Standards Institute recommendations. For evaluating the genotypic diversity, pulsed-field gel electrophoresis (PFGE) and\/or enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) was performed. The species identified were\n            <jats:named-content content-type=\"genus-species\">Mycobacterium abscessus<\/jats:named-content>\n            subsp.\n            <jats:named-content content-type=\"genus-species\">bolletii<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 24),\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            subsp.\n            <jats:named-content content-type=\"genus-species\">abscessus<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 6),\n            <jats:named-content content-type=\"genus-species\">Mycobacterium fortuitum<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 3),\n            <jats:named-content content-type=\"genus-species\">Mycobacterium marseillense<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 2), and\n            <jats:named-content content-type=\"genus-species\">Mycobacterium timonense<\/jats:named-content>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 1). Most of the isolates presented resistance to five or more of the antimicrobials tested. Typing profiles were mainly patient specific. The PFGE profiles indicated the presence of two clonal groups for\n            <jats:named-content content-type=\"genus-species\">M. abscessus<\/jats:named-content>\n            subsp.\n            <jats:named-content content-type=\"genus-species\">abscessus<\/jats:named-content>\n            and five clonal groups for\n            <jats:named-content content-type=\"genus-species\">M. abscesssus<\/jats:named-content>\n            subsp.\n            <jats:named-content content-type=\"genus-species\">bolletii<\/jats:named-content>\n            , with just one clone detected in two patients. Given the observed multidrug resistance patterns and the possibility of transmission between patients, we suggest the implementation of continuous and routine investigation of NTM infection or colonization in CF patients, including countries with a high burden of tuberculosis disease.\n          <\/jats:p>","DOI":"10.1128\/jcm.00549-14","type":"journal-article","created":{"date-parts":[[2014,6,12]],"date-time":"2014-06-12T08:21:53Z","timestamp":1402561313000},"page":"2990-2997","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Multidrug-Resistant Nontuberculous Mycobacteria Isolated from Cystic Fibrosis Patients"],"prefix":"10.1128","volume":"52","author":[{"given":"Pedro Henrique Campanini","family":"C\u00e2ndido","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Cl\u00ednica M\u00e9dica, Hospital Universit\u00e1rio Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana de Souza","family":"Nunes","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Ci\u00eancias M\u00e9dicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth Andrade","family":"Marques","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ci\u00eancias M\u00e9dicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e2nia Wrobel","family":"Folescu","sequence":"additional","affiliation":[{"name":"Instituto Fernandes Figueira, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1brice Santana","family":"Coelho","sequence":"additional","affiliation":[{"name":"Hospital Universit\u00e1rio Pedro Ernesto, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinicius Calado Nogueira","family":"de Moura","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlei Gomes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Machado","family":"Gomes","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina da Silva","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio Silva","family":"Aguiar","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s Gradua\u00e7\u00e3o em Cl\u00ednica M\u00e9dica, Hospital Universit\u00e1rio Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Chitolina","sequence":"additional","affiliation":[{"name":"Instituto de Doen\u00e7as do T\u00f3rax, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek T.","family":"Armstrong","sequence":"additional","affiliation":[{"name":"Johns Hopkins University, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia Cardoso","family":"Le\u00e3o","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Piedade Gon\u00e7alves","family":"Neves","sequence":"additional","affiliation":[{"name":"Instituto Biom\u00e9dico, Universidade Federal Fluminense, Niter\u00f3i, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Carvalho de Queiroz","family":"Mello","sequence":"additional","affiliation":[{"name":"Instituto de Doen\u00e7as do T\u00f3rax, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Silva","family":"Duarte","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"issue":"4","key":"e_1_3_2_2_2","first-page":"23","article-title":"Perfil 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A.","family":"Land","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,8]]},"references-count":34,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1128\/JCM.00549-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00549-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8]]},"assertion":[{"value":"2014-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-07-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}