{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T13:10:14Z","timestamp":1760015414631},"reference-count":45,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2015,5,1]],"date-time":"2015-05-01T00:00:00Z","timestamp":1430438400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Candida<\/jats:named-content>\n species are common causes of bloodstream infections (BSI), with high mortality. Four species cause >90% of\n Candida<\/jats:named-content>\n BSI:\n C. albicans<\/jats:named-content>\n ,\n C. glabrata<\/jats:named-content>\n ,\n C. parapsilosis<\/jats:named-content>\n , and\n C. tropicalis<\/jats:named-content>\n . Differentiation of\n Candida<\/jats:named-content>\n spp. is important because of differences in virulence and antimicrobial susceptibility.\n Candida<\/jats:named-content>\n QuickFISH BC, a multicolor, qualitative nucleic acid hybridization assay for the identification of\n C. albicans<\/jats:named-content>\n (green fluorescence),\n C. glabrata<\/jats:named-content>\n (red fluorescence), and\n C. parapsilosis<\/jats:named-content>\n (yellow fluorescence), was tested on Bactec and BacT\/Alert blood culture bottles which signaled positive on automated blood culture devices and were positive for yeast by Gram stain at seven study sites. The results were compared to conventional identification. A total of 419 yeast-positive blood culture bottles were studied, consisting of 258 clinical samples (89\n C. glabrata<\/jats:named-content>\n , 79\n C. albicans<\/jats:named-content>\n , 23\n C. parapsilosis<\/jats:named-content>\n , 18\n C. tropicalis<\/jats:named-content>\n , and 49 other species) and 161 contrived samples inoculated with clinical isolates (40\n C. glabrata<\/jats:named-content>\n , 46\n C. albicans<\/jats:named-content>\n , 36\n C. parapsilosis<\/jats:named-content>\n , 19\n C. tropicalis<\/jats:named-content>\n , and 20 other species). A total of 415 samples contained a single fungal species, with\n C. glabrata<\/jats:named-content>\n (\n n<\/jats:italic>\n = 129; 30.8%) being the most common isolate, followed by\n C. albicans<\/jats:named-content>\n (\n n<\/jats:italic>\n = 125; 29.8%),\n C. parapsilosis<\/jats:named-content>\n (\n n<\/jats:italic>\n = 59; 14.1%),\n C. tropicalis<\/jats:named-content>\n (\n n<\/jats:italic>\n = 37; 8.8%), and\n C. krusei<\/jats:named-content>\n (\n n<\/jats:italic>\n = 17; 4.1%). The overall agreement (with range for the three major\n Candida<\/jats:named-content>\n species) between the two methods was 99.3% (98.3 to 100%), with a sensitivity of 99.7% (98.3 to 100%) and a specificity of 98.0% (99.4 to 100%). This study showed that\n Candida<\/jats:named-content>\n QuickFISH BC is a rapid and accurate method for identifying\n C. albicans<\/jats:named-content>\n ,\n C. glabrata<\/jats:named-content>\n , and\n C. parapsilosis<\/jats:named-content>\n , the three most common\n Candida<\/jats:named-content>\n species causing BSI, directly from blood culture bottles.\n <\/jats:p>","DOI":"10.1128\/jcm.00549-15","type":"journal-article","created":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T02:22:55Z","timestamp":1426126975000},"page":"1672-1676","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Multicenter Evaluation of Candida QuickFISH BC for Identification of Candida Species Directly from Blood Culture Bottles"],"prefix":"10.1128","volume":"53","author":[{"given":"Ayman M.","family":"Abdelhamed","sequence":"first","affiliation":[{"name":"Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean X.","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Johns Hopkins Hospital and The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tonya","family":"Watkins","sequence":"additional","affiliation":[{"name":"The Johns Hopkins Hospital and The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margie A.","family":"Morgan","sequence":"additional","affiliation":[{"name":"Cedars Sinai Medical Center, Los Angeles, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fann","family":"Wu","sequence":"additional","affiliation":[{"name":"Columbia University Medical Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca J.","family":"Buckner","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DeAnna D.","family":"Fuller","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Davis","sequence":"additional","affiliation":[{"name":"Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Salimnia","sequence":"additional","affiliation":[{"name":"Wayne State University School of Medicine and Detroit Medical Center University Laboratory, Detroit, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilynn R.","family":"Fairfax","sequence":"additional","affiliation":[{"name":"Wayne State University School of Medicine and 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