{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:20:54Z","timestamp":1791091254984,"version":"4.1.0"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World\n            <jats:italic>Leishmania<\/jats:italic>\n            was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.\n          <\/jats:p>","DOI":"10.1128\/jcm.00553-10","type":"journal-article","created":{"date-parts":[[2010,4,15]],"date-time":"2010-04-15T01:57:18Z","timestamp":1271296638000},"page":"2240-2242","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Rapid Diagnosis of Old World Leishmaniasis by High-Resolution Melting Analysis of the 7SL RNA Gene"],"prefix":"10.1128","volume":"48","author":[{"given":"Abedelmajeed","family":"Nasereddin","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, the Kuvin Center for the Study of Infectious and Tropical Diseases, IMRIC, Hebrew University-Hadassah Medical School, P.O. 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