{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T17:01:22Z","timestamp":1792602082503,"version":"4.5.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Broad-range real-time PCR and sequencing of the 16S rRNA gene region is a widely known method for the detection and identification of bacteria in clinical samples. However, because of the need for sequencing, such identification of bacteria is time-consuming. The aim of our study was to develop a more rapid 16S real-time PCR-based identification assay using species- or genus-specific probes. The Gram-negative bacteria were divided into\n            <jats:italic>Pseudomonas<\/jats:italic>\n            species,\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            ,\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , and other Gram-negative species. Within the Gram-positive species, probes were designed for\n            <jats:italic>Staphylococcus<\/jats:italic>\n            species,\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            ,\n            <jats:italic>Enterococcus<\/jats:italic>\n            species,\n            <jats:italic>Streptococcus<\/jats:italic>\n            species, and\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            . The assay also included a universal probe within the 16S rRNA gene region for the detection of all bacterial DNA. The assay was evaluated with a collection of 248 blood cultures. In this study, the universal probe and the probes targeting\n            <jats:italic>Pseudomonas<\/jats:italic>\n            spp.,\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            ,\n            <jats:italic>E. coli<\/jats:italic>\n            ,\n            <jats:italic>Streptococcus<\/jats:italic>\n            spp.,\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            ,\n            <jats:italic>Enterococcus<\/jats:italic>\n            spp., and\n            <jats:italic>Staphylococcus<\/jats:italic>\n            spp. all had a sensitivity and specificity of 100%. The probe specific for\n            <jats:italic>S. aureus<\/jats:italic>\n            showed eight discrepancies, resulting in a sensitivity of 100% and a specificity of 93%. These data showed high agreement between conventional testing and our novel real-time PCR assay. Furthermore, this assay significantly reduced the time needed for identification. In conclusion, using pathogen-specific probes offers a faster alternative for pathogen detection and could improve the diagnosis of bloodstream infections.\n          <\/jats:p>","DOI":"10.1128\/jcm.00562-10","type":"journal-article","created":{"date-parts":[[2010,10,21]],"date-time":"2010-10-21T02:33:08Z","timestamp":1287628388000},"page":"4432-4438","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Molecular Probes for Diagnosis of Clinically Relevant Bacterial Infections in Blood Cultures"],"prefix":"10.1128","volume":"48","author":[{"given":"Wendy L. J.","family":"Hansen","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Maastricht University Medical Center, and the Care and Public Health Research Institute (CAPHRI), Maastricht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Beuving","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Maastricht University Medical Center, and the Care and Public Health Research Institute (CAPHRI), Maastricht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathrien A.","family":"Bruggeman","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Maastricht University Medical Center, and the Care and Public Health Research Institute (CAPHRI), Maastricht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra F. G.","family":"Wolffs","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Maastricht University Medical Center, and the Care and Public Health Research Institute (CAPHRI), Maastricht, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3119-3125.2003"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-008-0672-6"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-9-126"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-008-0747-5"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00821-09"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47732-0"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.05.009"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-148-1-257"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01205-8"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.10.3578-3582.2001"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61569-5"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2009.03.001"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00491-09"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02940.x"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00567-09"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02736.x"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00562-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00562-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:36:44Z","timestamp":1646469404000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00562-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":16,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["10.1128\/JCM.00562-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00562-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12]]},"assertion":[{"value":"2010-03-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-10-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}