{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T00:09:48Z","timestamp":1772755788971,"version":"3.50.1"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Trichomoniasis is a significant sexually transmitted disease (STD) in the spectrum of public health and primary care because of its association with agents such as human immunodeficiency virus and\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            . However, its true significance may be underestimated due to diagnostic modalities that exhibit poor sensitivity. A total of 1,086 genital specimens from two urban emergency departments, a suburban urgent-care facility, and a metropolitan outpatient physician group were subjected to transcription-mediated amplification-based\n            <jats:italic>Trichomonas vaginalis<\/jats:italic>\n            analyte-specific-reagent (ASR) testing (Gen-Probe, Inc.). The rate of positive molecular ASR results (14.5%) doubled that of direct saline preparation (7.0%;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0002). Analogous increases were observed at one emergency department and within the outpatient physician group (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0002). No significant increase in the rate of positive molecular ASR results was observed from the facilities that encountered a lower frequency of black\/African American patients. While positive\n            <jats:italic>T. vaginalis<\/jats:italic>\n            findings via direct saline preparation did not have a significant association with concomitant\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            or\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            infection overall, a positive\n            <jats:italic>T. vaginalis<\/jats:italic>\n            ASR result was a better predictor of concomitant\n            <jats:italic>C. trachomatis<\/jats:italic>\n            or\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            infection (odds ratios of 2.34 and 4.46, respectively;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001). The increased rate of positive\n            <jats:italic>T. vaginalis<\/jats:italic>\n            ASR results was observed in both point-of-care (\n            <jats:italic>P<\/jats:italic>\n            = 0.02 versus direct saline preparation) and laboratory (\n            <jats:italic>P<\/jats:italic>\n            = 0.003) testing. Highly sensitive\n            <jats:italic>T. vaginalis<\/jats:italic>\n            molecular ASR not only transcends issues of specimen integrity and microscopic acumen but also has an increased ability to predict the likelihood of additional STDs in defined populations.\n          <\/jats:p>","DOI":"10.1128\/jcm.00564-08","type":"journal-article","created":{"date-parts":[[2008,8,28]],"date-time":"2008-08-28T00:54:47Z","timestamp":1219884887000},"page":"3368-3374","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Impact of\n            <i>Trichomonas vaginalis<\/i>\n            Transcription-Mediated Amplification-Based Analyte-Specific-Reagent Testing in a Metropolitan Setting of High Sexually Transmitted Disease Prevalence"],"prefix":"10.1128","volume":"46","author":[{"given":"Erik","family":"Munson","sequence":"first","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"},{"name":"College of Health Sciences, University of Wisconsin\u2014Milwaukee, Milwaukee, Wisconsin 53201"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maureen","family":"Napierala","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin","family":"Olson","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina","family":"Endes","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Block","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne E.","family":"Hryciuk","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan and Midwest Clinical Laboratories, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald F.","family":"Schell","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene"},{"name":"Departments of Bacteriology"},{"name":"Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01121.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.1.148-152.1994"},{"key":"e_1_3_2_4_2","first-page":"9.6.6.1","year":"2004","unstructured":"Bruckner, D. 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