{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:23:41Z","timestamp":1792477421004,"version":"4.5.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Lpf (stands for long polar fimbriae) is one of the few adhesive factors of enterohemorrhagic\n            <jats:italic>Escherichia coli<\/jats:italic>\n            O157:H7 associated with colonization of the intestine.\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 strains possess two\n            <jats:italic>lpf<\/jats:italic>\n            loci encoding highly regulated fimbrial structures. Database analysis of the genes encoding the major fimbrial subunits demonstrated that they are present in commensal as well as pathogenic (both intestinal and extraintestinal)\n            <jats:italic>E. coli<\/jats:italic>\n            strains and in\n            <jats:italic>Salmonella<\/jats:italic>\n            strains and that the\n            <jats:italic>lpfA1<\/jats:italic>\n            and\n            <jats:italic>lpfA2<\/jats:italic>\n            genes are highly prevalent among LEE (locus of enterocyte effacement)-positive\n            <jats:italic>E. coli<\/jats:italic>\n            strains associated with severe and\/or epidemic disease. Further DNA sequence analysis of the\n            <jats:italic>lpfA1<\/jats:italic>\n            and\n            <jats:italic>lpfA2<\/jats:italic>\n            genes from different attaching-and-effacing\n            <jats:italic>E. coli<\/jats:italic>\n            strains has led us to the identification of several polymorphisms and the classification of the major fimbrial subunits into distinct variants. Using collections of pathogenic\n            <jats:italic>E. coli<\/jats:italic>\n            isolates from Europe and Latin America, we demonstrated that the different\n            <jats:italic>lpfA<\/jats:italic>\n            types are associated with the presence of specific intimin (\n            <jats:italic>eae<\/jats:italic>\n            ) adhesin variants and, most importantly, that they are found in specific\n            <jats:italic>E. coli<\/jats:italic>\n            pathotypes. Our results showed that the use of these fimbrial genes as markers, in combination with the different intimin types, resulted in a specific test for the identification of\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7, distinguishing it from other pathogenic\n            <jats:italic>E. coli<\/jats:italic>\n            strains.\n          <\/jats:p>","DOI":"10.1128\/jcm.00566-09","type":"journal-article","created":{"date-parts":[[2009,6,4]],"date-time":"2009-06-04T01:35:55Z","timestamp":1244079355000},"page":"2442-2451","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Genes Related to Long Polar Fimbriae of Pathogenic\n            <i>Escherichia coli<\/i>\n            Strains as Reliable Markers To Identify Virulent Isolates"],"prefix":"10.1128","volume":"47","author":[{"given":"Alfredo G.","family":"Torres","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology"},{"name":"Department of Pathology and Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, Texas 77555-1070"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Blanco","sequence":"additional","affiliation":[{"name":"Departamento de Microbioloxi\u0301a e Parasitoloxi\u0301a, Universidade de Santiago de Compostela, Lugo, Spain 27002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricio","family":"Valenzuela","sequence":"additional","affiliation":[{"name":"Programa de Microbiologi\u0301a, Instituto de Ciencias Biome\u0301dicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry M.","family":"Slater","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilpa D.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghizlane","family":"Dahbi","sequence":"additional","affiliation":[{"name":"Departamento de Microbioloxi\u0301a e Parasitoloxi\u0301a, Universidade de Santiago de Compostela, Lugo, Spain 27002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Lo\u0301pez","sequence":"additional","affiliation":[{"name":"Departamento de Microbioloxi\u0301a e Parasitoloxi\u0301a, Universidade de Santiago de Compostela, Lugo, Spain 27002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ximena Ferna\u0301ndez","family":"Barriga","sequence":"additional","affiliation":[{"name":"Programa de Microbiologi\u0301a, Instituto de Ciencias Biome\u0301dicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesu\u0301s E.","family":"Blanco","sequence":"additional","affiliation":[{"name":"Departamento de Microbioloxi\u0301a e Parasitoloxi\u0301a, Universidade de Santiago de Compostela, Lugo, Spain 27002"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u0302nia A. 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