{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T16:01:56Z","timestamp":1790352116041,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Diagnosis of invasive pulmonary aspergillosis (IPA) remains a major challenge to clinical microbiology laboratories. We developed rapid and sensitive quantitative PCR (qPCR) assays for genus- and species-specific identification of\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            infections by use of TaqMan technology. In order to validate these assays and understand their potential diagnostic utility, we then performed a blinded study of bronchoalveolar lavage (BAL) fluid specimens from well-characterized models of IPA with the four medically important species. A set of real-time qPCR primers and probes was developed by utilizing unique ITS1 regions for genus- and species-specific detection of the four most common medically important\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            species (\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">A. flavus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">A. niger<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">A. terreus<\/jats:named-content>\n            ). Pan-\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            and species-specific qPCRs with BAL fluid were more sensitive than culture for detection of IPA caused by\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            in untreated (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0007) and treated (\n            <jats:italic>P<\/jats:italic>\n            \u2264 0.008) animals, respectively. For infections caused by\n            <jats:named-content content-type=\"genus-species\">A. terreus<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">A. niger<\/jats:named-content>\n            , culture and PCR amplification from BAL fluid yielded similar sensitivities for untreated and treated animals. Pan-\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            PCR was more sensitive than culture for detection of\n            <jats:named-content content-type=\"genus-species\">A. flavus<\/jats:named-content>\n            in treated animals (\n            <jats:italic>P<\/jats:italic>\n            = 0.002). BAL fluid pan-\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            and species-specific PCRs were comparable in sensitivity to BAL fluid galactomannan (GM) assay. The copy numbers from the qPCR assays correlated with quantitative cultures to determine the pulmonary residual fungal burdens in lung tissue. Pan-\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            and species-specific qPCR assays may improve the rapid and accurate identification of IPA in immunocompromised patients.\n          <\/jats:p>","DOI":"10.1128\/jcm.00570-11","type":"journal-article","created":{"date-parts":[[2011,10,6]],"date-time":"2011-10-06T00:53:59Z","timestamp":1317862439000},"page":"4150-4157","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["Molecular Detection and Species-Specific Identification of Medically Important Aspergillus Species by Real-Time PCR in Experimental Invasive Pulmonary Aspergillosis"],"prefix":"10.1128","volume":"49","author":[{"given":"Thomas J.","family":"Walsh","sequence":"first","affiliation":[{"name":"Immunocompromised Host Section, Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, 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Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven B.","family":"Kleiboeker","sequence":"additional","affiliation":[{"name":"Viracor-IBT Laboratories, Lee's Summit, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushruth K.","family":"Reddy","sequence":"additional","affiliation":[{"name":"Viracor-IBT Laboratories, Lee's Summit, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01070-09"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00077-07"},{"key":"e_1_3_1_4_2","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1086\/588660","article-title":"Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer\/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group 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