{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T04:33:00Z","timestamp":1781325180641,"version":"3.54.1"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            There is a need for a point-of-care serodiagnostic test for women and men for sexually transmitted infections (STIs) caused by\n            <jats:named-content content-type=\"genus-species\">Trichomonas vaginalis<\/jats:named-content>\n            . Sera from women with this STI and sera from men that were analyzed in studies showing a relationship between serostatus and prostate cancer are highly seropositive in response to trichomonad \u03b1-actinin and its truncated protein (ACT-P2) (positive control sera). Epitope mapping experiments showed that positive control sera from women had antibodies to 13 distinct epitopes, 5 of which were detected by positive control sera from men. Sera from women and men that were unreactive with \u03b1-actinin (negative control sera) failed to detect any of the epitopes or other \u03b1-actinin amino acid sequences. The\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            \u03b1-actinin amino acid sequence and the sequences of the epitopes showed little or no identity with those of other proteins of microbial pathogens or the human \u03b1-actinin 1 (HuACTN1) homolog. Immunoassays such as dot blot, immunoblot, and enzyme-linked immunosorbent assays were used. Positive control sera did not detect HuACTN1 in immunoassays, and the range of levels of identity of \u03b1-actinin epitopes with HuACTN1 was 0% to 50%. Comparison of the\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            \u03b1-actinin epitopes with proteins in data banks, such as\n            <jats:named-content content-type=\"genus-species\">Tritrichomonas suis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Candida albicans<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n            proteins, gave a range of identity levels of 0% to 22%. Specific 15-mer peptide epitopes of \u03b1-actinin with low to no identity with other proteins were synthesized and were reactive with positive control sera only. These findings identify epitopes of \u03b1-actinin as candidate serodiagnostic targets and suggest strongly that a highly seropositive reaction to \u03b1-actinin suggests exposure to\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.00582-13","type":"journal-article","created":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T04:14:37Z","timestamp":1366863277000},"page":"2483-2490","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Epitopes of the Highly Immunogenic Trichomonas vaginalis \u03b1-Actinin Are Serodiagnostic Targets for Both Women and Men"],"prefix":"10.1128","volume":"51","author":[{"given":"Calvin J.","family":"Neace","sequence":"first","affiliation":[{"name":"School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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