{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:41:21Z","timestamp":1790286081149,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T00:00:00Z","timestamp":1729036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"HHS | National Institutes of Health","award":["U01CK000437, U01CK000580"],"award-info":[{"award-number":["U01CK000437, U01CK000580"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2024,10,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>The Advisory Committee on Immunization Practices (ACIP) recommended that dengue pre-vaccination screening tests for Dengvaxia administration have at least 98% specificity and 75% sensitivity. This study evaluates the performance of commercial anti-DENV IgG tests to identify tests that could be used for pre-vaccination screening. First, for seven tests, we evaluated sensitivity and specificity in early convalescent dengue virus (DENV) infection, using 44 samples collected 7\u201330 days after symptom onset and confirmed by RT-PCR. Next, for the five best-performing tests and two additional tests (with and without an external test reader) that became available later, we evaluated performance to detect past dengue infection among a panel of 44 specimens collected in 2018\u20132019 from healthy 9- to 16-year-old children from Puerto Rico. Finally, a full-scale evaluation was done with the four best-performing tests using 400 specimens from the same population. We used virus focus reduction neutralization test and an in-house DENV IgG ELISA as reference standards. Of seven tests, five showed \u226575% sensitivity in detecting anti-DENV IgG in early convalescent specimens with low cross-reactivity to the Zika virus. For the detection of previous DENV infections, the tests with the highest performance were the Euroimmun NS1 IgG ELISA (sensitivity 84.5%, specificity 97.1%) and CTK Dengue IgG rapid test R0065C with the test reader (sensitivity 76.2% specificity 98.1%). There are IgG tests available that can be used to accurately classify individuals with previous DENV infection as eligible for dengue vaccination to support safe vaccine implementation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>The Advisory Committee on Immunization Practices (ACIP) has set forth recommendations that dengue pre-vaccination screening tests must exhibit at least 98% specificity and 75% sensitivity. Our research rigorously assesses the performance of various commercial tests against these benchmarks using well-characterized specimens from Puerto Rico. The findings from our study are particularly relevant given FDA approval and ACIP recommendation of Sanofi Pasteur\u2019s Dengvaxia vaccine, highlighting the need for accurate pre-vaccination screening tools.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/jcm.00593-24","type":"journal-article","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T13:03:45Z","timestamp":1724850225000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Comparison of the sensitivity and specificity of commercial anti-dengue virus IgG tests to identify persons eligible for dengue vaccination"],"prefix":"10.1128","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1320-593X","authenticated-orcid":false,"given":"Freddy A.","family":"Medina","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0635-3010","authenticated-orcid":false,"given":"Frances","family":"Vila","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura E.","family":"Adams","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Cardona","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Carrion","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine","family":"Lamirande","sequence":"additional","affiliation":[{"name":"National Institutes of Health (NIH), Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz N.","family":"Acosta","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos M.","family":"De Le\u00f3n-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Beltran","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demian","family":"Grau","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Rivera-Amill","sequence":"additional","affiliation":[{"name":"Ponce Health Sciences University\/Ponce Research Institute, Ponce, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Balmaseda","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional de Virolog\u00eda, Centro Nacional de Diagn\u00f3stico y Referencia, Ministerio de Salud, Managua, Nicaragua"},{"name":"Sustainable Sciences Institute, Managua, Nicaragua"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7238-4037","authenticated-orcid":false,"given":"Eva","family":"Harris","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Vaccinology, School of Public Health, University of California, Berkeley, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary J.","family":"Madewell","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Waterman","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, Puerto Rico, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Paz-Bailey","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention (CDC), San Juan, 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for Disease Control and Prevention. 2022. Laboratory testing requirements for vaccination with Dengvaxia dengue vaccine (for healthcare providers). Available from: https:\/\/www.cdc.gov\/dengue\/vaccine\/hcp\/testing.html. Retrieved 18 Nov 2022."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-018-3274-7"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-023-05661-w"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00593-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00593-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T13:01:39Z","timestamp":1729083699000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.00593-24"}},"subtitle":[],"editor":[{"given":"Bobbi S.","family":"Pritt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,10,16]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10,16]]}},"alternative-id":["10.1128\/jcm.00593-24"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00593-24","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,16]]},"assertion":[{"value":"2024-04-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00593-24"}}