{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:43:22Z","timestamp":1790833402531,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Cryptosporidium meleagridis<\/jats:named-content>is a common cause of cryptosporidiosis in avian hosts and the third most common species involved in human cryptosporidiosis. Sequencing of the highly polymorphic 60-kDa glycoprotein (gp60) gene is a frequently used tool for investigation of the genetic diversity and transmission dynamics of<jats:named-content content-type=\"genus-species\">Cryptosporidium<\/jats:named-content>. However, few studies have included gp60 sequencing of<jats:named-content content-type=\"genus-species\">C. meleagridis<\/jats:named-content>. One explanation may be that the gp60 primers currently in use are based on<jats:named-content content-type=\"genus-species\">Cryptosporidium hominis<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">Cryptosporidium parvum<\/jats:named-content>sequence data, potentially limiting successful amplification of the<jats:named-content content-type=\"genus-species\">C. meleagridis<\/jats:named-content>gp60 gene. We therefore aimed to design primers for better gp60 subtyping of<jats:named-content content-type=\"genus-species\">C. meleagridis<\/jats:named-content>. Initially, \u223c1,440 bp of the gp60 locus of seven<jats:named-content content-type=\"genus-species\">C. meleagridis<\/jats:named-content>isolates were amplified using primers flanking the open reading frame. The obtained sequence data (\u223c1,250 bp) were used to design primers for a nested PCR targeting<jats:named-content content-type=\"genus-species\">C. meleagridis<\/jats:named-content>. Twenty isolates (16 from human and 4 from poultry) previously identified as<jats:named-content content-type=\"genus-species\">C. meleagridis<\/jats:named-content>by analysis of small subunit (SSU) rRNA genes were investigated. Amplicons of the expected size (\u223c900 bp) were obtained from all 20 isolates. The subsequent sequence analysis identified 3 subtype families and 10 different subtypes. The most common subtype family, IIIb, was identified in 12 isolates, represented by 6 subtypes, 4 new and 2 previously reported. Subtype family IIIe was found in 3 isolates represented by 3 novel, distinct subtypes. Finally, IIIgA31G3R1 was found in 1 human isolate and 4 poultry isolates, all originating from a previously reported<jats:named-content content-type=\"genus-species\">C. meleagridis<\/jats:named-content>outbreak at a Swedish organic farm.<\/jats:p>","DOI":"10.1128\/jcm.00598-14","type":"journal-article","created":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T02:02:23Z","timestamp":1397700143000},"page":"2311-2319","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["High Applicability of a Novel Method for gp60-Based Subtyping of Cryptosporidium meleagridis"],"prefix":"10.1128","volume":"52","author":[{"given":"Christen Rune","family":"Stensvold","sequence":"first","affiliation":[{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Beser","sequence":"additional","affiliation":[{"name":"Public Health Agency of Sweden, Solna, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Ax\u00e9n","sequence":"additional","affiliation":[{"name":"National Veterinary Institute, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Lebbad","sequence":"additional","affiliation":[{"name":"Public Health Agency of Sweden, Solna, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60844-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.4415\/ANN_12_04_10"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.02.003"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-391429-3.00007-1"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268812001665"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2003.tb00620.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/519842"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1179\/000349802125002202"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.03.018"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0368-1742(55)80025-2"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.8.3386-3391.1999"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.2.735-738.2000"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1180-1183.2000"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb09228.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054857"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4117-4134.2000"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2744-2747.2003"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.7.4108-4116.2000"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2001.tb00439.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.08.002"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.02.022"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2805-2809.2005"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.08.008"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268811000860"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12425"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.02.017"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02758-12"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1086\/318090"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2008.10.016"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0164-5"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2009.08.003"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3881-3890.2002"},{"key":"e_1_3_3_35_2","doi-asserted-by":"crossref","first-page":"214","DOI":"10.7883\/yoken.63.214","article-title":"Genotype analysis of Cryptosporidium meleagridis isolates from humans in Japan","volume":"63","author":"Abe N","year":"2010","unstructured":"AbeN. 2010. 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