{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T23:33:42Z","timestamp":1782862422214,"version":"3.54.5"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["5UM1 AI 106707"],"award-info":[{"award-number":["5UM1 AI 106707"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Nucleic acid amplification testing (NAAT) is the preferred method to detect\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            , but no commercial tests are cleared by the U.S. Food and Drug Administration for use with extragenital swab samples. This study evaluated the performance of the Gen-Probe Aptima Combo2 assay (Aptima) and the Cepheid Xpert CT\/NG assay (Xpert) to detect\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            in rectal and pharyngeal samples from 224 men and 175 women reporting a history of anal receptive sexual intercourse. Discordant results between the NAATs were repeated using the assays APTIMA CT or APTIMA GC, which target alternate primers, as the confirmatory tests.\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            was detected from 59 rectal swabs and 8 pharyngeal samples, with 97.7% and 99.5% agreement between the two test systems, respectively. For\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            , Xpert was 95% sensitive (95% CI, 86 to 99%) and Aptima was 92% sensitive (95% CI, 81 to 97%) from rectal swabs, while both systems were 100% sensitive from pharyngeal samples.\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            was detected from 30 rectal and 40 pharyngeal samples, with 99.5% and 97.5% agreement between the two test systems. The sensitivity of Xpert for\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            from rectal swabs was 100% (95% CI, 88 to 100%) versus 93% (95% CI, 78 to 99%) for Aptima. From pharyngeal swab samples, Xpert was 98% sensitive (95% CI, 87 to 99.9%) versus 93% (95% CI, 80 to 98%) for Aptima. For\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            , neither system was &gt;95% sensitive from the rectum, though both were &gt;99.5% specific. For\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            , Xpert had higher sensitivity than Aptima, but with more false positives from pharyngeal samples.\n          <\/jats:p>","DOI":"10.1128\/jcm.00616-17","type":"journal-article","created":{"date-parts":[[2017,7,6]],"date-time":"2017-07-06T01:00:23Z","timestamp":1499302823000},"page":"2801-2807","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Use of Nucleic Acid Amplification Testing for Diagnosis of Extragenital Sexually Transmitted Infections"],"prefix":"10.1128","volume":"55","author":[{"given":"Lisa A.","family":"Cosentino","sequence":"first","affiliation":[{"name":"Magee-Womens Research Institute, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire S.","family":"Danby","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorna K.","family":"Rabe","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Macio","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie A.","family":"Meyn","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold C.","family":"Wiesenfeld","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.","family":"Hillier","sequence":"additional","affiliation":[{"name":"Magee-Womens Research Institute, Pittsburgh, Pennsylvania, USA"},{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1","article-title":"Sexually transmitted diseases treatment guidelines, 2015","volume":"64","author":"Centers for Disease Control and Prevention","year":"2015","unstructured":"Centers for Disease Control and Prevention. 2015. Sexually transmitted diseases treatment guidelines, 2015. MMWR Morb Mortal Wkly Rep64:1\u2013138.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000553"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00185-12"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31817bdd7e"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01581-08"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2008.034140"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02398-09"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw594"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/430704"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31821890e9"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu184"},{"key":"e_1_3_2_13_2","first-page":"716","article-title":"Clinic-based testing for rectal and pharyngeal Neisseria gonorrhoeae and Chlamydia trachomatis Infections by community-based organizations\u2014five cities, United States, 2007","volume":"58","author":"Centers for Disease Control and Prevention","year":"2009","unstructured":"Centers for Disease Control and Prevention. 2009. Clinic-based testing for rectal and pharyngeal Neisseria gonorrhoeae and Chlamydia trachomatis Infections by community-based organizations\u2014five cities, United States, 2007. MMWR Morb Mortal Wkly Rep58(26):716\u2013719.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181bcd3e7"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000384"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2014.871495"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01930-12"},{"issue":"1","key":"e_1_3_2_18_2","first-page":"021","article-title":"Validation of the GeneXpert\u00ae CT\/NG assay for use with male pharyngeal and rectal swabs","volume":"3","author":"Geiger R","year":"2016","unstructured":"Geiger R Smith DM Little SJ Mehta SR . 2016. Validation of the GeneXpert\u00ae CT\/NG assay for use with male pharyngeal and rectal swabs. Austin J HIV AIDS Res3(1). pii:021.","journal-title":"Austin J HIV AIDS Res"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01217-11"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00806-13"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3182495e2f"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2015-052502"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2015.09.002"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03461-12"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00616-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00616-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:58:45Z","timestamp":1646470725000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00616-17"}},"subtitle":[],"editor":[{"given":"Erik","family":"Munson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,9]]},"references-count":23,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1128\/JCM.00616-17"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00616-17","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9]]},"assertion":[{"value":"2017-04-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-06-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}