{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:18:39Z","timestamp":1790468319872,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2013,6,19]],"date-time":"2013-06-19T00:00:00Z","timestamp":1371600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"},{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Droplet digital PCR (ddPCR) is an emulsion PCR process that performs absolute quantitation of nucleic acids. We developed a ddPCR assay for\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            infections and found it to be accurate and precise. Using PCR mixtures containing plasmids engineered to include the PCR target sequences, we were able to quantify with a dynamic range between 0.07 and 3,160 targets\/\u03bcl (\n            <jats:italic>r<\/jats:italic>\n            <jats:sup>2<\/jats:sup>\n            = 0.9927) with &gt;95% confidence. Using 1,509 clinical conjunctival swab samples from a population in which trachoma is endemic in Guinea Bissau, we evaluated the specificity and sensitivity of the quantitative ddPCR assay in diagnosing ocular\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            infections by comparing the performances of ddPCR and the Roche Amplicor CT\/NG test. We defined ddPCR tests as positive when we had \u226595% confidence in a nonzero estimate of target load. The sensitivity of ddPCR against Amplicor was 73.3% (95% confidence interval [CI], 67.9 to 78.7%), and specificity was 99.1% (95% CI, 98.6 to 99.6%). Negative and positive predictive values were 94.6% (95% CI, 93.4 to 95.8%) and 94.5% (95% CI, 91.3 to 97.7%), respectively. Based on Amplicor CT\/NG testing, the estimated population prevalence of\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            ocular infection was \u223c17.5%. Receiver-operator curve analysis was used to select critical cutoff values for use in clinical settings in which a balance between higher sensitivity and specificity is required. We concluded that ddPCR is an effective diagnostic technology suitable for both research and clinical use in diagnosing ocular\n            <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n            infections.\n          <\/jats:p>","DOI":"10.1128\/jcm.00622-13","type":"journal-article","created":{"date-parts":[[2013,5,2]],"date-time":"2013-05-02T07:43:15Z","timestamp":1367480595000},"page":"2195-2203","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["Development and Evaluation of a Next-Generation Digital PCR Diagnostic Assay for Ocular Chlamydia trachomatis Infections"],"prefix":"10.1128","volume":"51","author":[{"given":"Chrissy h.","family":"Roberts","sequence":"first","affiliation":[{"name":"Clinical Research Department, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Last","sequence":"additional","affiliation":[{"name":"Clinical Research Department, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Molina-Gonzalez","sequence":"additional","affiliation":[{"name":"Clinical Research Department, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunice","family":"Cassama","sequence":"additional","affiliation":[{"name":"Programa Nacional de Saude de Visao, Ministerio de Saude Publica, Bissau, Guinea Bissau"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Butcher","sequence":"additional","affiliation":[{"name":"Clinical Research Department, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meno","family":"Nabicassa","sequence":"additional","affiliation":[{"name":"Programa Nacional de Saude de Visao, Ministerio de Saude Publica, Bissau, Guinea Bissau"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Department of Immunology, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E.","family":"Burr","sequence":"additional","affiliation":[{"name":"Clinical Research Department, London School of Hygiene and Tropical Medicine, London, United Kingdom"},{"name":"Medical Research Council Unit, The Gambia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C.","family":"Mabey","sequence":"additional","affiliation":[{"name":"Clinical Research Department, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin L.","family":"Bailey","sequence":"additional","affiliation":[{"name":"Clinical Research Department, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin J.","family":"Holland","sequence":"additional","affiliation":[{"name":"Clinical Research Department, London School of Hygiene and Tropical Medicine, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"161","article-title":"Global WHO alliance for the elimination of blinding trachoma by 2020","volume":"87","author":"WHO","year":"2012","unstructured":"WHO. 2012. 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