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In combination with clinical symptoms and radiographic findings, antibody-based diagnostic tests are often used to diagnose and monitor patients with VF. Chitinase 1 (CTS1) has previously been identified as the seroreactive antigen used in these diagnostic assays to detect anticoccidial IgG. Here, an indirect enzyme-linked immunosorbent assay to detect IgG to CTS1 demonstrated 165 of 178 (92.7%) patients with a positive result by immunodiffusion (ID) and\/or complement fixation (CF) had antibodies to the single antigen CTS1. We then developed a rapid antibody lateral flow assay (LFA) to detect anti-CTS1 antibodies. Out of 143 samples tested, the LFA showed 92.9% positive percent agreement [95% confidence interval (CI), 84.3%\u201396.9%] and 97.7% negative percent agreement (95% CI, 87.9%\u201399.6%) with ID and CF assays. Serum or plasma from canines, macaques, and dolphins was also tested by the CTS1 LFA. Test line densities of the CTS1 LFA correlated in a linear manner with the reported CF and ID titers for human and non-human samples, respectively. This 10-min point-of-care test for the rapid detection of anti-coccidioidal antibodies could help to inform healthcare providers in real-time, potentially improving the efficiency of healthcare delivery.<\/jats:p>","DOI":"10.1128\/jcm.00631-23","type":"journal-article","created":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T13:01:15Z","timestamp":1693573275000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Development of a rapid lateral flow assay for detection of anti-coccidioidal antibodies"],"prefix":"10.1128","volume":"61","author":[{"given":"Francisca J.","family":"Grill","sequence":"first","affiliation":[{"name":"School of Life Sciences, Arizona State University , Tempe, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergei","family":"Svarovsky","sequence":"additional","affiliation":[{"name":"Glycodots LLC , San Diego, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Gonzalez-Moa","sequence":"additional","affiliation":[{"name":"Glycodots LLC , San Diego, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin","family":"Kaleta","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic , Phoenix, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janis E.","family":"Blair","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Mayo Clinic , Phoenix, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia","family":"Lovato","sequence":"additional","affiliation":[{"name":"Veterinary Neurological Center , Phoenix, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Grant","sequence":"additional","affiliation":[{"name":"Washington National Primate Research Center , Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyle","family":"Ross","sequence":"additional","affiliation":[{"name":"National Marine Mammal Foundation , San Diego, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara K.","family":"Linnehan","sequence":"additional","affiliation":[{"name":"National Marine Mammal Foundation , San Diego, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny","family":"Meegan","sequence":"additional","affiliation":[{"name":"National Marine Mammal Foundation , San Diego, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenta S.","family":"Reilly","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, Mayo Clinic , Phoenix, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashlyn","family":"Brown","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Mayo Clinic , Phoenix, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacy","family":"Williams","sequence":"additional","affiliation":[{"name":"Center for Personalized Diagnostics, Biodesign Institute, Arizona State University , Tempe, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunro","family":"Chung","sequence":"additional","affiliation":[{"name":"Center for Personalized Diagnostics, Biodesign Institute, Arizona State University , Tempe, Arizona, USA"},{"name":"College of Health Solutions, Arizona State University , Phoenix, Arizona, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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