{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:20:09Z","timestamp":1770222009852,"version":"3.49.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:named-content content-type=\"genus-species\">Cytauxzoon felis<\/jats:named-content>is a virulent, tick-transmitted, protozoan parasite that infects felines. Cytauxzoonosis was previously thought to be uniformly fatal in domestic cats. Treatment combining atovaquone and azithromycin (A&amp;A) has been associated with survival rates of over 60%. Atovaquone, a ubiquinone analogue, targets<jats:named-content content-type=\"genus-species\">C. felis<\/jats:named-content>cytochrome<jats:italic>b<\/jats:italic>(<jats:italic>cytb<\/jats:italic>), of which 30 unique genotypes have been identified. The<jats:named-content content-type=\"genus-species\">C. felis<\/jats:named-content><jats:italic>cytb<\/jats:italic>genotype<jats:italic>cytb1<\/jats:italic>is associated with increased survival rates in cats treated with A&amp;A. The purpose of this study was to design a PCR panel that could distinguish<jats:named-content content-type=\"genus-species\">C. felis cytb1<\/jats:named-content>from other cytochrome<jats:italic>b<\/jats:italic>genotypes. Primer pairs were designed to span five different nucleotide positions at which single-nucleotide polymorphisms in the<jats:named-content content-type=\"genus-species\">C. felis cytb<\/jats:named-content>gene had been identified. Through the use of high-resolution melt analysis, this panel was predicted to distinguish<jats:italic>cytb1<\/jats:italic>from other<jats:italic>cytb<\/jats:italic>genotypes. Assays were validated using samples from 69 cats with cytauxzoonosis for which the<jats:named-content content-type=\"genus-species\">C. felis cytb<\/jats:named-content>genotypes had been characterized previously. The PCR panel identified<jats:named-content content-type=\"genus-species\">C. felis cytb1<\/jats:named-content>with 100% sensitivity and 98.2% specificity. High-resolution melt analysis can rapidly provide prognostic information for clients considering A&amp;A treatment in cats with cytauxzoonosis.<\/jats:p>","DOI":"10.1128\/jcm.00635-15","type":"journal-article","created":{"date-parts":[[2015,5,28]],"date-time":"2015-05-28T12:54:13Z","timestamp":1432817653000},"page":"2517-2524","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Rapid High-Resolution Melt Analysis of Cytauxzoon felis Cytochrome<i>b<\/i>To Aid in the Prognosis of Cytauxzoonosis"],"prefix":"10.1128","volume":"53","author":[{"given":"Megan E.","family":"Schreeg","sequence":"first","affiliation":[{"name":"North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry S.","family":"Marr","sequence":"additional","affiliation":[{"name":"North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime L.","family":"Tarigo","sequence":"additional","affiliation":[{"name":"North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leah A.","family":"Cohn","sequence":"additional","affiliation":[{"name":"University of Missouri, College of Veterinary Medicine, Columbia, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael G.","family":"Levy","sequence":"additional","affiliation":[{"name":"North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam J.","family":"Birkenheuer","sequence":"additional","affiliation":[{"name":"North Carolina State University, College of Veterinary Medicine, Raleigh, North Carolina, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"822","DOI":"10.2460\/javma.1984.184.07.822","article-title":"Cytauxzoonosis in domestic cats: report of two cases in Oklahoma, with a review and discussion of the disease","volume":"184","author":"Glenn BL","year":"1984","unstructured":"Glenn BL, Stair EL. 1984. Cytauxzoonosis in domestic cats: report of two cases in Oklahoma, with a review and discussion of the disease. J Am Vet Med Assoc 184:822\u2013825.","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"455","DOI":"10.2460\/javma.1994.205.03.455","article-title":"Cytauxzoonosis in cats: eight cases (1985\u20131992)","volume":"205","author":"Hoover JP","year":"1994","unstructured":"Hoover JP, Walker DB, Hedges JD. 1994. Cytauxzoonosis in cats: eight cases (1985\u20131992). J Am Vet Med Assoc 205:455\u2013460.","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"585","DOI":"10.2460\/javma.1976.168.07.585","article-title":"A fatal cytauxzoonosis-like disease in cats","volume":"168","author":"Wagner JE","year":"1976","unstructured":"Wagner JE. 1976. A fatal cytauxzoonosis-like disease in cats. J Am Vet Med Assoc 168:585\u2013588.","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-9571(79)90028-6"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.228.4.568"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.02.039"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-45.1.234"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X14567158"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2007.02.029"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-014-0618-z"},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.2460\/javma.1995.206.09.1363","article-title":"Survival of a domestic cat with naturally acquired cytauxzoonosis","volume":"206","author":"Walker DB","year":"1995","unstructured":"Walker DB, Cowell RL. 1995. Survival of a domestic cat with naturally acquired cytauxzoonosis. J Am Vet Med Assoc 206:1363\u20131365.","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2000.tb02270.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.04.041"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2010.0646.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"497","DOI":"10.2460\/javma.1999.215.04.497","article-title":"Administration of diminazene aceturate or imidocarb dipropionate for treatment of cytauxzoonosis in cats","volume":"215","author":"Greene CE","year":"1999","unstructured":"Greene CE, Latimer K, Hopper E, Shoeffler G, Lower K, Cullens F. 1999. Administration of diminazene aceturate or imidocarb dipropionate for treatment of cytauxzoonosis in cats. J Am Vet Med Assoc 215:497\u2013500.","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_3_3_17_2","volume-title":"Infectious diseases of the dog and cat","author":"Greene CE","year":"1998","unstructured":"Greene CE. 1998. Infectious diseases of the dog and cat, 2nd ed. W. B. Saunders, Philadelphia, PA.","edition":"2"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.2174\/138945007779315632"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.091208.073424"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2009.0300.x"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.8.2100-2108.2000"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2007.12.002"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01407-13"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2011.06.001"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/292017"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2008.03982.x"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2006.11.025"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.61.1.1-16.1997"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01709-12"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.21019"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1586\/erm.09.84"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.21003"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2008.01.010"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.21004"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2012.49"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.12.007"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a112510"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00635-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00635-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T03:53:30Z","timestamp":1748404410000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00635-15"}},"subtitle":[],"editor":[{"given":"B. W.","family":"Fenwick","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2015,8]]},"references-count":36,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8]]}},"alternative-id":["10.1128\/JCM.00635-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00635-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8]]},"assertion":[{"value":"2015-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}