{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T10:24:39Z","timestamp":1789208679502,"version":"build-2803163510"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The mycobacterium growth indicator tube (MGIT960) automated liquid medium testing method is becoming the international gold standard for second-line drug susceptibility testing of multidrug- and extensively drug-resistant\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            complex isolates. We performed a comparative study of the current gold standard in the Netherlands, the Middlebrook 7H10 agar dilution method, the MGIT960 system, and the GenoType MTBDR\n            <jats:italic>sl<\/jats:italic>\n            genotypic method for rapid screening of aminoglycoside and fluoroquinolone resistance. We selected 28 clinical multidrug- and extensively drug-resistant\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            complex strains and\n            <jats:italic>M. tuberculosis<\/jats:italic>\n            H37Rv. We included amikacin, capreomycin, moxifloxacin, prothionamide, clofazimine, linezolid, and rifabutin in the phenotypic test panels. For prothionamide and moxifloxacin, the various proposed breakpoint concentrations were tested by using the MGIT960 method. The MGIT960 method yielded results 10 days faster than the agar dilution method. For amikacin, capreomycin, linezolid, and rifabutin, results obtained by all methods were fully concordant. Applying a breakpoint of 0.5 \u03bcg\/ml for moxifloxacin led to results concordant with those of both the agar dilution method and the genotypic method. For prothionamide, concordance was noted only at the lowest and highest MICs. The phenotypic methods yielded largely identical results, except for those for prothionamide. Our study supports the following breakpoints for the MGIT960 method: 1 \u03bcg\/ml for amikacin, linezolid, and clofazimine, 0.5 \u03bcg\/ml for moxifloxacin and rifabutin, and 2.5 \u03bcg\/ml for capreomycin. No breakpoint was previously proposed for clofazimine. For prothionamide, a division into susceptible, intermediate, and resistant seems warranted, although the boundaries require additional study. The genotypic assay proved a reliable and rapid method for predicting aminoglycoside and fluoroquinolone resistance.\n          <\/jats:p>","DOI":"10.1128\/jcm.00652-10","type":"journal-article","created":{"date-parts":[[2010,6,17]],"date-time":"2010-06-17T01:56:56Z","timestamp":1276739816000},"page":"2749-2753","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Comparative Study on Genotypic and Phenotypic Second-Line Drug Resistance Testing of\n            <i>Mycobacterium tuberculosis<\/i>\n            Complex Isolates"],"prefix":"10.1128","volume":"48","author":[{"given":"Jakko","family":"van Ingen","sequence":"first","affiliation":[{"name":"National Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands"},{"name":"Department of Pulmonary Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sami","family":"Simons","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rina","family":"de Zwaan","sequence":"additional","affiliation":[{"name":"National Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tridia","family":"van der Laan","sequence":"additional","affiliation":[{"name":"National Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miranda","family":"Kamst-van Agterveld","sequence":"additional","affiliation":[{"name":"National Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin J.","family":"Boeree","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Diseases, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dick","family":"van Soolingen","sequence":"additional","affiliation":[{"name":"National Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0176-6724(87)80043-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00671-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-9792(08)70002-7"},{"key":"e_1_3_2_5_2","unstructured":"GenoType MTBDRsl manual v1.0. 2009"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00081-09"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00089808"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.811-818.2006"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3192-3197.2005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/521894"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-004-1109-5"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.08.018"},{"key":"e_1_3_2_13_2","first-page":"1449","volume":"12","year":"2008","unstructured":"Rodrigues, C., J. Jani, S. Shenai, P. Thakkar, S. Siddiqi, and A. Mehta. 2008. Drug susceptibility testing of Mycobacterium tuberculosis against second-line drugs using the BACTEC MGIT 960 system. Int. J. Tuberc. Lung Dis. 12 : 1449-1455.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.688-692.2006"},{"key":"e_1_3_2_15_2","first-page":"128","volume":"12","year":"2008","unstructured":"Rustomjee, R., C. Lienhardt, T. Kanyok, G. R. Davies, J. Levin, T. Mthiyane, C. Reddy, A. W. Sturm, F. A. Sirgel, J. Allen, D. J. Coleman, B. Fourie, D. A. Mitchison, et al. 2008. A phase II study of the sterilising activities of ofloxacin, gatifloxacin and moxifloxacin in pulmonary tuberculosis. Int. J. Tuberc. Lung Dis. 12 : 128-138.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00414-06"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02501-08"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2004.16.4.334"},{"key":"e_1_3_2_19_2","first-page":"1295","volume":"12","year":"2008","unstructured":"van Ingen, J., M. J. Boeree, A. Wright, T. van der Laan, P. N. R. Dekhuijzen, and D. van Soolingen. 2008. Second-line drug resistance in multi-drug resistant tuberculosis cases of various origins in the Netherlands. Int. J. Tuberc. Lung Dis. 12 : 1295-1299.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2009.09.023"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00652-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00652-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:19:46Z","timestamp":1646471986000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00652-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8]]},"references-count":19,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["10.1128\/JCM.00652-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00652-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,8]]},"assertion":[{"value":"2010-03-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-06-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-08-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}