{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T21:46:41Z","timestamp":1789076801155,"version":"build-2803163510"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rapid isothermal amplification methods have recently been introduced, and some of these methods offer significant advantages over PCR. The objective of this study was to develop a rapid and sensitive multiplex loop-mediated isothermal amplification (M-LAMP) assay for the detection of respiratory syncytial virus subgroups A and B (RSV A and B). We designed six primers each for the matrix gene of RSV A and the polymerase gene of RSV B and developed an M-LAMP assay by using a commercially available master mix and a real-time fluorometer (Genie II; Optigene, United Kingdom) that displays real-time amplification, time to positivity, and amplicon annealing temperature (\n            <jats:italic>\n              T\n              <jats:sub>m<\/jats:sub>\n            <\/jats:italic>\n            ). The M-LAMP was evaluated against PCR by testing 275 nasopharyngeal (NP) specimens. The final optimized M-LAMP assay had a mean amplification time of 14.2 min (compared with 90 to 120 min for PCR) and had an analytical sensitivity of 1 genome equivalent (ge) for both RSV A and B. Using PCR as a comparator, M-LAMP had a sensitivity of 100% (81\/81) and specificity of 100% (194\/194). We also evaluated a 3- to 10-min specimen processing method involving vortexing with glass beads and heating to 98\u00b0C in M-swab medium (Copan Italia, Brescia, Italy) and found that this rapid processing method allowed detection of 37\/41 (90.2%) of positives when we used extracted nucleic acid. In summary, the M-LAMP assay had excellent sensitivity and specificity for detecting RSV A and B in NP specimens and, when coupled with a rapid specimen preparation method, could provide a specimen-to-result diagnosis time of 30 min.\n          <\/jats:p>","DOI":"10.1128\/jcm.00662-13","type":"journal-article","created":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T03:53:26Z","timestamp":1371095606000},"page":"2696-2701","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Development of a Sensitive Loop-Mediated Isothermal Amplification Assay That Provides Specimen-to-Result Diagnosis of Respiratory Syncytial Virus Infection in 30 Minutes"],"prefix":"10.1128","volume":"51","author":[{"given":"James","family":"Mahony","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Medicine, McMaster University"},{"name":"Regional Virology and Chlamydiology Laboratory, St. Joseph's Healthcare Hamilton, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Chong","sequence":"additional","affiliation":[{"name":"Regional Virology and Chlamydiology Laboratory, St. Joseph's Healthcare Hamilton, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Bulir","sequence":"additional","affiliation":[{"name":"Regional Virology and Chlamydiology Laboratory, St. Joseph's Healthcare Hamilton, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Ruyter","sequence":"additional","affiliation":[{"name":"Regional Virology and Chlamydiology Laboratory, St. Joseph's Healthcare Hamilton, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Mwawasi","sequence":"additional","affiliation":[{"name":"Regional Virology and Chlamydiology Laboratory, St. Joseph's Healthcare Hamilton, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Waltho","sequence":"additional","affiliation":[{"name":"Regional Virology and Chlamydiology Laboratory, St. Joseph's Healthcare Hamilton, Hamilton, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00037-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.10.121"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1283288"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cll.2009.07.005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408363.2011.640976"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2011.01.007"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2002.0415"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2006.03.003"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-360"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20424"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2006.09.014"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.2144\/0000113902"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152612800100099"},{"key":"e_1_3_2_16_2","first-page":"1","volume-title":"Respiratory syncytial virus","author":"Gilleland J","year":"2012","unstructured":"GillelandJ MahonyJ PaesB. 2012. Respiratory syncytial virus infection: diagnostic investigation, management and preventive strategies, p 1\u201378. In NamH GippeumK (ed), Respiratory syncytial virus. Nova Sciences Publishers Inc., New York, NY."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152612800100116"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/S10096-006-0112-4"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.05.030"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001357"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2009.066688"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(07)70007-0"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.5.1796-1801.2001"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2008.04.011"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-12-15"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506848103"},{"key":"e_1_3_2_27_2","article-title":"Molecular detection of respiratory viruses: where do we go from here?","author":"Mahony JB","unstructured":"MahonyJB. Molecular detection of respiratory viruses: where do we go from here? Eur. Infect. Dis. Rev., in press.","journal-title":"Eur. Infect. Dis. Rev"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bioeng.10.061807.160524"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0lc00666a"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00662-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00662-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:45:29Z","timestamp":1646480729000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00662-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":28,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1128\/JCM.00662-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00662-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8]]},"assertion":[{"value":"2013-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}