{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T08:05:54Z","timestamp":1788595554348,"version":"build-2803163510"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Blastomyces dermatitidis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Histoplasma capsulatum<\/jats:named-content>\n            are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by these fungi may have very similar clinical presentations. Although microbiologic culture is a standard method for detecting these pathogens, their recovery may require days to weeks, and the manipulation of cultures presents a significant safety hazard to laboratory personnel. Therefore, the goal of this study was to design a rapid, real-time PCR assay to detect and differentiate\n            <jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            from culture isolates and directly from clinical specimens. Primers and fluorescence resonance energy transfer hybridization probes were designed to target the histidine kinase and glyceraldehyde-3-phosphate dehydrogenase genes of\n            <jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            , respectively. The analytical sensitivity of the assay was determined to be 100 copies\/\u03bcl for both fungi. From culture isolates, the assay demonstrated 100% specificity and 100% sensitivity for\n            <jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>\n            and 100% specificity and 94% sensitivity for\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            . Detection directly from 797 clinical specimens demonstrated specificities and sensitivities of 99% and 86% for\n            <jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>\n            and 100% and 73% for\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            compared with the results for culture. This real-time PCR assay provides a rapid method for the detection of\n            <jats:named-content content-type=\"genus-species\">B. dermatitidis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">H. capsulatum<\/jats:named-content>\n            from culture isolates and directly from clinical specimens.\n          <\/jats:p>","DOI":"10.1128\/jcm.00673-11","type":"journal-article","created":{"date-parts":[[2011,7,14]],"date-time":"2011-07-14T02:57:40Z","timestamp":1310612260000},"page":"3204-3208","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":125,"title":["Detection of Blastomyces dermatitidis and Histoplasma capsulatum from Culture Isolates and Clinical Specimens by Use of Real-Time PCR"],"prefix":"10.1128","volume":"49","author":[{"given":"N. Esther","family":"Babady","sequence":"first","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seanne P.","family":"Buckwalter","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie","family":"Hall","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kara M.","family":"Le Febre","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Binnicker","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy L.","family":"Wengenack","sequence":"additional","affiliation":[{"name":"Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Abstr. 109th Gen. 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