{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:26:26Z","timestamp":1791152786951,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Molecular evidence is limited for the hypothesis that humans, dogs, and cats can become colonized and infected with similar virulent<jats:italic>Escherichia coli<\/jats:italic>strains. To further assess this possibility, archived<jats:italic>E. coli<\/jats:italic>O6 isolates (<jats:italic>n<\/jats:italic>= 130) from humans (<jats:italic>n<\/jats:italic>= 55), dogs (<jats:italic>n<\/jats:italic>= 59), and cats (<jats:italic>n<\/jats:italic>= 16), representing the three main H (flagellar) types within serogroup O6 (H1, H7, and H31), were analyzed, along with selected reference strains. Isolates underwent PCR-based phylotyping, multilocus sequence typing, PCR-based detection of 55 virulence-associated genes, and XbaI pulsed-field gel electrophoresis (PFGE) profiling. Three major sequence types (STs), which corresponded closely with H types, accounted for 99% of the 130 O6 isolates. Each ST included human, dog, and cat isolates; two included reference pyelonephritis isolates CFT073 (O6:K2:H1) and 536 (O6:K15:H31). Virulence genotypes overlapped considerably among host species, despite statistically significant differences between human and pet isolates. Several human and dog isolates from ST127 (O6:H31) exhibited identical virulence genotypes and highly similar PFGE profiles, consistent with cross-species exchange of specific<jats:italic>E. coli<\/jats:italic>clones. In conclusion, the close similarity in the genomic backbone and virulence genotype between certain human- and animal-source<jats:italic>E. coli<\/jats:italic>isolates within serogroup O6 supports the hypothesis of zoonotic potential.<\/jats:p>","DOI":"10.1128\/jcm.00674-07","type":"journal-article","created":{"date-parts":[[2007,11,15]],"date-time":"2007-11-15T01:54:48Z","timestamp":1195091688000},"page":"417-422","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Virulence Genotypes and Phylogenetic Background of<i>Escherichia coli<\/i>Serogroup O6 Isolates from Humans, Dogs, and Cats"],"prefix":"10.1128","volume":"46","author":[{"given":"James R.","family":"Johnson","sequence":"first","affiliation":[{"name":"Mucosal and Vaccine Research Center"},{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Johnston","sequence":"additional","affiliation":[{"name":"Mucosal and Vaccine Research Center"},{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connie R.","family":"Clabots","sequence":"additional","affiliation":[{"name":"Mucosal and Vaccine Research Center"},{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Kuskowski","sequence":"additional","affiliation":[{"name":"Geriatric Research, Education, and Clinical Center, Veterans Affairs Medical Center"},{"name":"Psychiatry, University of Minnesota, Minneapolis, Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Roberts","sequence":"additional","affiliation":[{"name":"E. coli Reference Center, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chitrita","family":"DebRoy","sequence":"additional","affiliation":[{"name":"E. coli Reference Center, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"285","volume":"30","year":"1999","unstructured":"Beutin, L. 1999. 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