{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:00:07Z","timestamp":1791338407050,"version":"4.3.1"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Between June and November 2010, a concerning rise in the number of cases of puerperal sepsis, a postpartum pelvic bacterial infection contracted by women after childbirth, was observed in the New South Wales, Australia, hospital system. Group A streptococcus (GAS;\n            <jats:named-content content-type=\"genus-species\">Streptococcus pyogenes<\/jats:named-content>\n            ) isolates PS001 to PS011 were recovered from nine patients. Pulsed-field gel electrophoresis and\n            <jats:italic>emm<\/jats:italic>\n            sequence typing revealed that GAS of\n            <jats:italic>emm1.40<\/jats:italic>\n            ,\n            <jats:italic>emm75.0<\/jats:italic>\n            ,\n            <jats:italic>emm77.0<\/jats:italic>\n            ,\n            <jats:italic>emm89.0<\/jats:italic>\n            , and\n            <jats:italic>emm89.9<\/jats:italic>\n            were each recovered from a single patient, ruling out a single source of infection. However,\n            <jats:italic>emm28.8<\/jats:italic>\n            GAS were recovered from four different patients. To investigate the relatedness of these\n            <jats:italic>emm28<\/jats:italic>\n            isolates, whole-genome sequencing was undertaken and the genome sequences were compared to the genome sequence of the\n            <jats:italic>emm28.4<\/jats:italic>\n            reference strain, MGAS6180. A total of 186 single nucleotide polymorphisms were identified, for which the phylogenetic reconstruction indicated an outbreak of a polyclonal nature. While two isolates collected from different hospitals were not closely related, isolates from two puerperal sepsis patients from the same hospital were indistinguishable, suggesting patient-to-patient transmission or infection from a common source. The results of this study indicate that traditional typing protocols, such as pulsed-field gel electrophoresis, may not be sensitive enough to allow fine epidemiological discrimination of closely related bacterial isolates. Whole-genome sequencing presents a valid alternative that allows accurate fine-scale epidemiological investigation of bacterial infectious disease.\n          <\/jats:p>","DOI":"10.1128\/jcm.00675-12","type":"journal-article","created":{"date-parts":[[2012,4,20]],"date-time":"2012-04-20T00:13:41Z","timestamp":1334880821000},"page":"2224-2228","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Analysis of a Streptococcus pyogenes Puerperal Sepsis Cluster by Use of Whole-Genome Sequencing"],"prefix":"10.1128","volume":"50","author":[{"given":"Nouri L.","family":"Ben Zakour","sequence":"first","affiliation":[{"name":"School of Chemistry and Molecular Biosciences and Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carola","family":"Venturini","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences and Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Beatson","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences and Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark J.","family":"Walker","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Biosciences and Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-402"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3000713"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.4.953-958.1996"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000800"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01378-10"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti553"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/342062"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1198545"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1086\/519936"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011147"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.2002.tb05004.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1985.tb123000.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.4083-4091.2005"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/430618"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1182395"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8603-7-14"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0266-6138(05)80039-7"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/318710"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2010.01.012"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ogx.0000265998.40912.5e"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3844-3846.2004"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268807008746"},{"key":"e_1_3_3_27_2","unstructured":"NSWHealth. 2010. Safety Alert Broadcast System. Safety notice SN:017\/10. NSW Government Sydney Australia. http:\/\/www.health.nsw.gov.au\/quality\/sabs. Accessed October 2011."},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-130-2-199901190-00006"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1106920"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1086\/502567"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009785"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-11-65"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01470.x"},{"key":"e_1_3_3_34_2","unstructured":"WHO. 2005. WHO guidelines on hand hygiene in health care (advanced draft): summary. Clean hands are safe hands. World Alliance for Patient Safety World Health Organization Geneva Switzerland."},{"key":"e_1_3_3_35_2","volume-title":"The global burden of disease: 2004 update","author":"WHO","year":"2008","unstructured":"WHO. 2008. The global burden of disease: 2004 update. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.074492.107"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00675-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00675-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:18:12Z","timestamp":1646479092000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00675-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7]]},"references-count":35,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2012,7]]}},"alternative-id":["10.1128\/JCM.00675-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00675-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7]]},"assertion":[{"value":"2012-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-04-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}