{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:47:39Z","timestamp":1792709259730,"version":"4.5.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Naturally acquired infection of humans with a marine mammal-associated<jats:italic>Brucella<\/jats:italic>sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of<jats:italic>Brucella<\/jats:italic>from a New Zealand patient that appears most closely related to strains previously identified from marine mammals. The isolate was preliminarily identified as<jats:italic>Brucella suis<\/jats:italic>using conventional bacteriological tests in our laboratory. However, the results profile was not an exact match, and the isolate was forwarded to four international reference laboratories for further identification. The reference laboratories identified the isolate as either<jats:italic>B. suis<\/jats:italic>or<jats:italic>B. melitensis<\/jats:italic>by traditional bacteriological methods in three laboratories and by a molecular test in the fourth laboratory. Molecular characterization by PCR, PCR-restriction fragment length polymorphism, and DNA sequencing of the<jats:italic>bp26<\/jats:italic>gene; IS<jats:italic>711<\/jats:italic>; the<jats:italic>omp<\/jats:italic>genes<jats:italic>omp25<\/jats:italic>,<jats:italic>omp31<\/jats:italic>,<jats:italic>omp2a<\/jats:italic>, and<jats:italic>omp2b<\/jats:italic>; IRS-PCR fragments I, III, and IV; and five housekeeping gene fragments was conducted to resolve the discrepant identification of the isolate. The isolate was identified to be closely related to a<jats:italic>Brucella<\/jats:italic>sp. originating from a United States bottlenose dolphin (<jats:italic>Tursiops truncatus<\/jats:italic>) and common seals (<jats:italic>Phoca vitulina<\/jats:italic>).<\/jats:p>","DOI":"10.1128\/jcm.00680-06","type":"journal-article","created":{"date-parts":[[2006,12,6]],"date-time":"2006-12-06T18:54:05Z","timestamp":1165431245000},"page":"4363-4370","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":148,"title":["Characterization of a<i>Brucella<\/i>sp. Strain as a Marine-Mammal Type despite Isolation from a Patient with Spinal Osteomyelitis in New Zealand"],"prefix":"10.1128","volume":"44","author":[{"given":"W. L.","family":"McDonald","sequence":"first","affiliation":[{"name":"Investigation and Diagnostic Centre, Biosecurity New Zealand, Ministry of Agriculture and Forestry, Ward St., Wallaceville, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Jamaludin","sequence":"additional","affiliation":[{"name":"Investigation and Diagnostic Centre, Biosecurity New Zealand, Ministry of Agriculture and Forestry, Ward St., Wallaceville, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Mackereth","sequence":"additional","affiliation":[{"name":"Investigation and Diagnostic Centre, Biosecurity New Zealand, Ministry of Agriculture and Forestry, Ward St., Wallaceville, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Investigation and Diagnostic Centre, Biosecurity New Zealand, Ministry of Agriculture and Forestry, Ward St., Wallaceville, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Humphrey","sequence":"additional","affiliation":[{"name":"Investigation and Diagnostic Centre, Biosecurity New Zealand, Ministry of Agriculture and Forestry, Ward St., Wallaceville, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Short","sequence":"additional","affiliation":[{"name":"Institute of Environmental Science and Research, Ltd., Kenepuru Science Centre, 34 Kenepuru Dr., Porirua, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Australian Animal Health Laboratory, Geelong, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Swingler","sequence":"additional","affiliation":[{"name":"Australian Animal Health Laboratory, Geelong, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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