{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T17:38:04Z","timestamp":1762623484992},"reference-count":20,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The sensitivity of microscopy for the diagnosis of tuberculosis (TB) is around 50% but decreases by about 15% in patients with suspected TB who are coinfected with HIV. Here, we compared the accuracies of three microscopy methods for processing sputum smears (concentration by centrifugation with or without\n            <jats:italic>N<\/jats:italic>\n            -acetyl-\n            <jats:sc>l<\/jats:sc>\n            -cysteine [NALC] and concentration by filtration on a polycarbonate membrane) to that of culture on Ogawa-Kudoh medium as the gold standard method. Sputum samples were obtained from 432 patients with suspected pulmonary TB, of whom 60% were infected with HIV. Analysis was performed using the first specimen. Compared to the gold standard culture, the small-membrane-filter (SMF) method was the most sensitive microscopic method. In HIV-infected TB patients, the sensitivity of the SMF method was significantly higher than those for centrifugation of sputum samples with or without NALC treatment (61.9%, 47.6%, and 45.2%, respectively;\n            <jats:italic>P<\/jats:italic>\n            = 0.001). Similarly, in TB patients without HIV infection, the sensitivity of the SMF method was significantly higher than those for centrifugation of sputum samples with or without NALC treatment (81.8%, 63.6%, and 57.5%, respectively;\n            <jats:italic>P<\/jats:italic>\n            = 0.001). In the two study groups, TB patients with or without HIV, no significant differences between the specificities of the three methods were observed. Handling of the second sputum sample similarly by centrifugation with or without NALC and by the SMF method increased positivities by 13%, 11%, and 4%, respectively. The overall agreement between microscopy and culture was above 90% for all groups. Microscopic evaluation of the sputum samples treated with NALC compared to those not treated with NALC did not show any increase in sensitivity. Altogether, the sensitivity of the SMF method is higher than those of the other two microscopic methods studied without a loss of specificity.\n          <\/jats:p>","DOI":"10.1128\/jcm.00683-13","type":"journal-article","created":{"date-parts":[[2013,6,27]],"date-time":"2013-06-27T04:23:55Z","timestamp":1372307035000},"page":"2921-2925","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Increased Sensitivity in Diagnosis of Tuberculosis in HIV-Positive Patients through the Small-Membrane-Filter Method of Microscopy"],"prefix":"10.1128","volume":"51","author":[{"given":"Patr\u00edcia","family":"Quinc\u00f3","sequence":"first","affiliation":[{"name":"Universidade do Estado do Amazonas\/P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Manaus, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samira","family":"B\u00fchrer-S\u00e9kula","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Heitor Vieira Dourado, Manaus, Amazonas, Brazil"},{"name":"Universidade Federal de Goi\u00e1s, Instituto de Patologia Tropical e Sa\u00fade P\u00fablica, Goi\u00e2nia, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walber","family":"Brand\u00e3o","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Heitor Vieira Dourado, Manaus, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rossiclea","family":"Monte","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Heitor Vieira Dourado, Manaus, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Leopoldina","family":"Souza","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Heitor Vieira Dourado, Manaus, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Saraceni","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o de Medicina Tropical Heitor Vieira Dourado, Manaus, Amazonas, Brazil"},{"name":"Secretaria Municipal de Sa\u00fade do Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moises","family":"Palaci","sequence":"additional","affiliation":[{"name":"N\u00facleo de Doen\u00e7as Infecciosas, Universidade Federal do Esp\u00edrito Santo, Vit\u00f3ria, ES, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reynaldo","family":"Dietze","sequence":"additional","affiliation":[{"name":"N\u00facleo de Doen\u00e7as Infecciosas, Universidade Federal do Esp\u00edrito Santo, Vit\u00f3ria, ES, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo","family":"Cordeiro-Santos","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas\/P\u00f3s-Gradua\u00e7\u00e3o em Medicina Tropical, Manaus, Amazonas, Brazil"},{"name":"Funda\u00e7\u00e3o de Medicina Tropical Heitor Vieira Dourado, Manaus, Amazonas, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir411"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00110210"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009859"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60284-0"},{"key":"e_1_3_2_6_2","first-page":"847","article-title":"\u201cSmear-negative\u201d pulmonary tuberculosis in a DOTS programme: poor outcomes in an area of high HIV seroprevalence","volume":"5","author":"Hargreaves NJ","year":"2001","unstructured":"HargreavesNJKadzakumanjaOWhittyCJSalaniponiFMHarriesADSquireSB. 2001. \u201cSmear-negative\u201d pulmonary tuberculosis in a DOTS programme: poor outcomes in an area of high HIV seroprevalence. Int. J. Tuberc. Lung Dis. 5:847\u2013854.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/518656"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00572-12"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.13.6.1109-1113.1981"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05835-11"},{"key":"e_1_3_2_11_2","unstructured":"Minist\u00e9rioda Sa\u00fadeSecretaria de Vigil\u00e2ncia emSa\u00fade Departamento de Vigil\u00e2ncia Epidemiol\u00f3gica. 11 December 2012 posting date. Manual nacional de vigil\u00e2ncia laboratorial da tuberculose e outras micobact\u00e9rias. Minist\u00e9rio da Sa\u00fade Brazil. http:\/\/portal.saude.gov.br\/portal\/arquivos\/pdf\/manual_laboratorio_tb_3_9_10.pdf."},{"key":"e_1_3_2_12_2","first-page":"71","article-title":"A simple technique for culturing tubercle bacilli","volume":"51","author":"Kudoh S","year":"1974","unstructured":"KudohSKudohT. 1974. A simple technique for culturing tubercle bacilli. Bull. World Health Organ. 51:71\u201382.","journal-title":"Bull. World Health Organ."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-8-68"},{"key":"e_1_3_2_14_2","first-page":"1130","article-title":"Sensitivity and specificity of fluorescence microscopy for diagnosing pulmonary tuberculosis in a high HIV prevalence setting","volume":"13","author":"Cattamanchi A","year":"2009","unstructured":"CattamanchiADavisJLWorodriaWden BoonSYooSMatovuJKiidhaJNankyaFKyeyuneRByanyimaPAndamaAJolobaMOsmondDHHopewellPCHuangL. 2009. Sensitivity and specificity of fluorescence microscopy for diagnosing pulmonary tuberculosis in a high HIV prevalence setting. Int. J. Tuberc. Lung Dis. 13:1130\u20131136.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-9-53"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(97)90263-7"},{"key":"e_1_3_2_17_2","first-page":"1163","article-title":"A comprehensive comparison of Ziehl-Neelsen and fluorescence microscopy for the diagnosis of tuberculosis in a resource-poor urban setting","volume":"7","author":"Kivihya-Ndugga LE","year":"2003","unstructured":"Kivihya-NduggaLEvan CleeffMRGithuiWANgangaLWKibugaDKOdhiamboJAKlatserPR. 2003. A comprehensive comparison of Ziehl-Neelsen and fluorescence microscopy for the diagnosis of tuberculosis in a resource-poor urban setting. Int. J. Tuberc. Lung Dis. 7:1163\u20131171.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70602-8"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD009593.pub2"},{"key":"e_1_3_2_20_2","unstructured":"Minist\u00e9rio da Sa\u00fade Secretaria de Vigil\u00e2ncia em Sa\u00fade Departamento de Vigil\u00e2ncia Epidemiol\u00f3gica Brazil. 11 December 2012 posting date. Manual de recomenda\u00e7\u00f5es para o controle da tuberculose no Brasil. Minist\u00e9rio da Sa\u00fade Brazil. http:\/\/portal.saude.gov.br\/portal\/arquivos\/pdf\/manual_de_recomendacoes_tb.pdf."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-9-821"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00683-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00683-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:32:10Z","timestamp":1646472730000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00683-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":20,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1128\/JCM.00683-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00683-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"2013-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}