{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:15:18Z","timestamp":1753881318202,"version":"3.41.2"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2024,9,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              This study evaluates the growth of mycobacteria in samples from cystic fibrosis (CF) patients and tissue samples using the mycobacteria growth indicator tube (MGIT) incubated at 30\u00b0C in comparison to conventional MGIT cultures incubated at 37\u00b0C in a BACTEC MGIT 960 device and solid media incubated at 36\u00b0C and 30\u00b0C. A total of 1,549 samples were analyzed, of which 202 mycobacterial isolates were cultured from 197 positive specimens, including five mixed cultures. The highest detection rate was achieved from MGIT at 30\u00b0C, with 84.2% of mycobacterial isolates (170 of 202), which was significantly higher than any other culture condition (\n              <jats:italic>P<\/jats:italic>\n              &lt; 0.0001 for any condition). MGIT at 37\u00b0C yielded 61.4% (124 of 202) of the recovered isolates, whereas L\u00f6wenstein Jensen (LJ) and Stonebrink at 36\u00b0C, and LJ and Stonebrink at 30\u00b0C retrieved 47.0% (95), 49.5% (100), 50.0% (101), and 53.0% (107) of the isolates, respectively. Of the 53 isolates that were grown exclusively under one culture condition, the highest number of isolates (36) was recovered from MGIT incubated at 30\u00b0C. MGIT at 37\u00b0C recovered eight of the 53 isolates, whereas LJ incubated at 30\u00b0C and Stonebrink incubated at 30\u00b0C and 36\u00b0C recovered five, three, and one isolate, respectively. No isolates were grown exclusively from LJ incubated at 36\u00b0C. In CF patients and tissue samples, MGIT cultivated at 30\u00b0C for 8 weeks increases the performance of mycobacterial culture.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Our study shows that the addition of mycobacteria growth indicator tube (MGIT) liquid culture incubated at 30\u00b0C improves the detection of mycobacteria from CF and tissue samples. MGIT incubated at 30\u00b0C recovered significantly more mycobacterial isolates than MGIT incubated at 37\u00b0C and significantly more isolates than either Lowenstein Jensen or Stonebrink solid media incubated at either 36\u00b0C or 30\u00b0C. Of 202 mycobacterial isolates recovered from 1,549 specimens, 170 were recovered from MGIT incubated at 30\u00b0C, followed by MGIT incubated at 37\u00b0C with 124 isolates and solid media culture conditions that recovered between 95 and 107 mycobacterial isolates. All conventional culture conditions combined without MGIT incubated at 30\u00b0C recovered 166 isolates. MGIT incubated at 30\u00b0C recovered the highest number of isolates detected exclusively by a single culture condition and recovered mycobacterial isolates of highly relevant mycobacterial species, including\n              <jats:italic>Mycobacterium abscessus<\/jats:italic>\n              and\n              <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n              .\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/jcm.00683-24","type":"journal-article","created":{"date-parts":[[2024,8,13]],"date-time":"2024-08-13T13:01:44Z","timestamp":1723554104000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Improved detection of mycobacteria in CF and tissue samples grown in mycobacteria growth indicator tube incubated at 30\u00b0C compared to conventional growth conditions of liquid and solid media"],"prefix":"10.1128","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1494-9191","authenticated-orcid":false,"given":"J.","family":"Kehrmann","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2114-3230","authenticated-orcid":false,"given":"A. L.","family":"Stumpf","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Dragaqina","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Buer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01528-20"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200604-571ST"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00957-10"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.3.748-752.1999"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.9.2236-2239.1996"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050492"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1309\/65KN-2M7E-7MNN-X0TA"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00038-17"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2015-207983"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12890-019-0781-2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01519-18"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.11.3761-3763.1999"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw778"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200207-678OC"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01257-09"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2023.01.032"},{"key":"e_1_3_2_18_2","first-page":"132","article-title":"The death and resurrection of the tubercle bacillus","volume":"67","author":"Loring WW","year":"1955","unstructured":"Loring WW, Melvin I, Vandiviere HM, Willis HS. 1955. The death and resurrection of the tubercle bacillus. Trans Am Clin Climatol Assoc 67:132\u2013138.","journal-title":"Trans Am Clin Climatol Assoc"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/11309"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.1029111"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.102.1.70"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00149212"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00683-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00683-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T13:07:19Z","timestamp":1726060039000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.00683-24"}},"subtitle":[],"editor":[{"given":"Christine Y.","family":"Turenne","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,9,11]]},"references-count":21,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9,11]]}},"alternative-id":["10.1128\/jcm.00683-24"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00683-24","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"type":"print","value":"0095-1137"},{"type":"electronic","value":"1098-660X"}],"subject":[],"published":{"date-parts":[[2024,9,11]]},"assertion":[{"value":"2024-05-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00683-24"}}