{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:04:13Z","timestamp":1770822253597,"version":"3.50.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,12,1]],"date-time":"2010-12-01T00:00:00Z","timestamp":1291161600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The microscopic observation drug susceptibility assay (MODS) is a novel and promising test for the early diagnosis of tuberculosis (TB). We evaluated the MODS assay for the early diagnosis of TB in HIV-positive patients presenting to Pham Ngoc Thach Hospital for Tuberculosis and Lung Diseases in southern Vietnam. A total of 738 consecutive sputum samples collected from 307 HIV-positive individuals suspected of TB were tested by smear, MODS, and the mycobacteria growth indicator tube method (MGIT). The diagnostic sensitivity and specificity of MODS compared to the microbiological gold standard (either smear or MGIT) were 87 and 93%, respectively. The sensitivities of smear, MODS, and MGIT were 57, 71, and 75%, respectively, against clinical gold standard (MODS versus smear,\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001; MODS versus MGIT,\n            <jats:italic>P<\/jats:italic>\n            = 0.03). The clinical gold standard was defined as patients who had a clinical examination and treatment consistent with TB, with or without microbiological confirmation. For the diagnosis of smear-negative patients, the sensitivities of MODS and MGIT were 38 and 45%, respectively (\n            <jats:italic>P<\/jats:italic>\n            = 0.08). The median times to detection using MODS and MGIT were 8 and 11 days, respectively, and they were 11 and 17 days, respectively, for smear-negative samples. The original bacterial\/fungal contamination rate of MODS was 1.1%, while it was 2.6% for MGIT. The cross-contamination rate of MODS was 4.7%. In conclusion, MODS is a sensitive, specific, and rapid test that is appropriate for the detection of HIV-associated TB; its cost and ease of use make it particularly useful in resource-limited settings.\n          <\/jats:p>","DOI":"10.1128\/jcm.00687-10","type":"journal-article","created":{"date-parts":[[2010,10,7]],"date-time":"2010-10-07T02:40:39Z","timestamp":1286419239000},"page":"4573-4579","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Diagnosis of Pulmonary Tuberculosis in HIV-Positive Patients by Microscopic Observation Drug Susceptibility Assay"],"prefix":"10.1128","volume":"48","author":[{"given":"Dang Thi Minh","family":"Ha","sequence":"first","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"},{"name":"Wellcome Trust Major Overseas Programme and Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Thi Ngoc","family":"Lan","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vo Sy","family":"Kiet","sequence":"additional","affiliation":[{"name":"Wellcome Trust Major Overseas Programme and Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Wolbers","sequence":"additional","affiliation":[{"name":"Wellcome Trust Major Overseas Programme and Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hoang Thi Thanh","family":"Hang","sequence":"additional","affiliation":[{"name":"Wellcome Trust Major Overseas Programme and Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Day","sequence":"additional","affiliation":[{"name":"Wellcome Trust Major Overseas Programme and Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Quang","family":"Hien","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Anh","family":"Tien","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pham Thuy","family":"An","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Truong Thi","family":"Anh","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Do Thi Tuong","family":"Oanh","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chau Luong","family":"Hoa","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Thi Minh","family":"Chau","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Ngoc","family":"Hai","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ngo Thanh","family":"Binh","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Le Hong","family":"Ngoc","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doan Thanh","family":"Phuong","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tran Van","family":"Quyet","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Thi Bich","family":"Tuyen","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vo Thi","family":"Ha","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Thi","family":"Nho","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dai Viet","family":"Hoa","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phan Thi Hoang","family":"Anh","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguyen Huy","family":"Dung","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach Hospital, 120 Hung Vuong, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Farrar","sequence":"additional","affiliation":[{"name":"Wellcome Trust Major Overseas Programme and Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxine","family":"Caws","sequence":"additional","affiliation":[{"name":"Wellcome Trust Major Overseas Programme and Oxford University Clinical Research Unit, Hospital for Tropical Diseases, 190 Ben ham Tu, District 5, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"287","volume":"67","year":"2007","unstructured":"Alonso, V., R. Paul, L. Barrera, and V. Ritacco. 2007. False diagnosis of tuberculosis by culture. Medicina 67 : 287-294. (In Spanish.)","journal-title":"Medicina"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/511639"},{"key":"e_1_3_2_4_2","unstructured":"J. Vis. Exp. 2008 11"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1502.080942"},{"key":"e_1_3_2_6_2","first-page":"707","volume":"362","year":"2009","unstructured":"Cain, K. P., K. D. McCarthy, C. M. Heilig, P. Monkongdee, T. Tasaneeyapan, N. Kanara, M. E. Kimerling, P. Chheng, S. Thai, B. Sar, P. Phanuphak, N. Teeratakulpisarn, N. Phanuphak, H. D. Nguyen, T. Q. Hoang, H. T. Le, and J. K. Varma. 2009. An algorithm for tuberculosis screening and diagnosis in people with HIV. N. Engl. J. Med. 362 : 707-716.","journal-title":"N. Engl. J. Med."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001173"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-9-821"},{"key":"e_1_3_2_9_2","first-page":"866","volume":"4","year":"2000","unstructured":"Chien, H. P., M. C. Yu, M. H. Wu, T. P. Lin, and K. T. Luh. 2000. Comparison of the BACTEC MGIT 960 with Lowenstein-Jensen medium for recovery of mycobacteria from clinical specimens. Int. J. Tuberc. Lung Dis. 4 : 866-870.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008341"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2323-2329.2003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2607-2618.1999"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0944-5013(99)80008-1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200905-0692OC"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa055524"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.12.4750-4752.2002"},{"key":"e_1_3_2_17_2","unstructured":"The statistical evaluation of medical tests for classification and prediction. 2004"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.916-919.1999"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/651081"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.5.1366-1369.1999"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.5.3.327"},{"key":"e_1_3_2_22_2","first-page":"1463","volume":"13","year":"2007","unstructured":"Tran, N. B., R. M. Houben, T. Q. Hoang, T. N. Nguyen, M. W. Borgdorff, and F. G. Cobelens. 2007. HIV and tuberculosis in Ho Chi Minh City, Vietnam, 1997-2002. Emerg. Infect. Dis. 13 : 1463-1469.","journal-title":"Emerg. Infect. Dis."},{"key":"e_1_3_2_23_2","unstructured":"World Health Organization. 2004. TB\/HIV: a clinical manual 2nd ed. World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/publications\/who_htm_tb_2004_329\/en\/index.html\n."},{"key":"e_1_3_2_24_2","unstructured":"World Health Organization. 2009. Global tuberculosis control 2009: epidemiology strategy and financing. World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/publications\/global_reprt\/2009\/\/pdf\/full_report.pdf\n."},{"key":"e_1_3_2_25_2","unstructured":"World Health Organization. 2009. Global tuberculosis control 2009: epidemiology strategy and financing (update). World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/publications\/global_reprt\/2009\/update\/tbu_9.pdf\n."},{"key":"e_1_3_2_26_2","unstructured":"World Health Organization. 2007. Improving the diagnosis and treatment of smear-negative pulmonary and extrapulmonary tuberculosis among adults and adolescents: recommendations for HIV-prevalent and resource constrained settings. World Health Organization Geneva Switzerland. http:\/\/whqlibdoc.who.int\/hq\/2007\/WHO_HTM_TB_2007.379_eng.pdf\n."},{"key":"e_1_3_2_27_2","unstructured":"World Health Organization. 2010. Laboratory service in tuberculosis control. II. Microscopy. WHO\/TB\/98.258. World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/publications\/1998\/en\/index2.html\n."},{"key":"e_1_3_2_28_2","unstructured":"World Health Organization. 2007. Use of liquid TB culture and drug susceptibility testing (DST) in low- and middle-income countries. Summary report of the expert group meeting on the use of liquid culture media. World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/dots\/laboratory\/Use%20of%20Liquid%20TB%20Culture_Summary%20Report.pdf\n."},{"key":"e_1_3_2_29_2","unstructured":"World Health Organization. 2008. WHO policy statement: molecular line probe assays for rapid screening of patients at risk of multidrug-resistant tuberculosis. World Health Organization Geneva Switzerland. http:\/\/www.who.int\/tb\/dots\/laboratory\/line_probe_assays\/en\/\n."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00687-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00687-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:34:10Z","timestamp":1646469250000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00687-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12]]},"references-count":28,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2010,12]]}},"alternative-id":["10.1128\/JCM.00687-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00687-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12]]},"assertion":[{"value":"2010-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-09-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}