{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:10:44Z","timestamp":1788959444159,"version":"build-2803163510"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21 AI182845"],"award-info":[{"award-number":["R21 AI182845"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["KL2 TR002385"],"award-info":[{"award-number":["KL2 TR002385"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["T32 AI007613"],"award-info":[{"award-number":["T32 AI007613"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2026,9,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Patients undergoing hematopoietic cell transplantation (HCT) who receive fluoroquinolone prophylaxis are at high risk for bacteremia due to fluoroquinolone-resistant viridans group streptococci (FQ-R VGS), particularly\n                      <jats:italic toggle=\"yes\">Streptococcus mitis<\/jats:italic>\n                      . Detecting colonization with FQ-R VGS may identify neutropenic patients at risk for streptococcal bacteremia during FQ prophylaxis, but the optimal specimen screening site and microbiologic screening method are unknown. We therefore simultaneously collected buccal, oropharyngeal, and perianal swabs from patients upon admission for HCT, and performed selective culture with both direct plating and broth enrichment. FQ-R VGS were detected in 62 (90%) of 69 participants. Direct plating resulted in a positive percent agreement (PPA) of 79% (95% confidence interval [CI]: 67%\u201388%) for detecting FQ-R VGS from buccal swabs, and a PPA of 86% (95% CI: 74%\u201393%) from oropharyngeal swabs compared to detection by any specimen or method. Broth enrichment yielded a PPA of 97% (95% CI: 89%\u2013100%) for both buccal and oropharyngeal swabs. Perianal swabs had low PPA. For detecting FQ-R\n                      <jats:italic toggle=\"yes\">S. mitis<\/jats:italic>\n                      group, the direct plating PPA was 74% (95% CI: 60%\u201385%) for buccal and 72% (95% CI: 58%\u201384%) for oropharyngeal swabs, while the broth enrichment PPA was 82% (95% CI: 69%\u201391%) and 58% (95% CI: 43%\u201372%), respectively. Combining direct plating and broth enrichment of buccal swabs had a PPA of 96% (95% CI: 86%\u2013100%) for detecting FQ-R\n                      <jats:italic toggle=\"yes\">S. mitis<\/jats:italic>\n                      group. Buccal swabs are a reliable specimen to detect FQ-R VGS colonization when using broth enrichment, and combining direct plating and broth enrichment may optimize detection of FQ-R\n                      <jats:italic toggle=\"yes\">S. mitis<\/jats:italic>\n                      group.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Viridans group streptococci (VGS) are important causes of bloodstream infections in patients with cancer undergoing hematopoietic cell transplantation. These infections frequently occur despite using prophylactic fluoroquinolone antibiotics, suggesting that some patients are colonized with fluoroquinolone-resistant VGS (FQ-R VGS). A test that reliably detects carriage of FQ-R VGS might help identify patients at highest risk of streptococcal infection, but the optimal methods to detect FQ-R VGS colonization were previously unknown. In this study, we compared different body sites and culture methods for detecting colonization with FQ-R VGS. We found that buccal (cheek) swabs reliably detected these resistant bacteria, including members of the\n                        <jats:italic toggle=\"yes\">Streptococcus mitis<\/jats:italic>\n                        group, and adding a broth enrichment step further improved detection from buccal samples. These findings support buccal sampling with broth enrichment as a practical approach for detecting colonization with FQ-R VGS and establish a foundation for future studies to define the clinical significance of FQ-R VGS colonization.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.00687-26","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:00:38Z","timestamp":1787310038000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Optimizing methods to detect colonization with fluoroquinolone-resistant viridans group streptococci prior to hematopoietic cell transplantation"],"prefix":"10.1128","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3488-4217","authenticated-orcid":false,"given":"Supram 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