{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T04:15:05Z","timestamp":1768968905548,"version":"3.49.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A duplex PCR (dPCR) assay was developed to simultaneously detect and differentiate\n            <jats:italic>Bartonella quintana<\/jats:italic>\n            ,\n            <jats:italic>Bartonella henselae<\/jats:italic>\n            , and\n            <jats:italic>Coxiella burnetii<\/jats:italic>\n            from surgical heart valve tissue specimens with an analytic sensitivity of 10 copies\/reaction. Among 17 specimens collected from patients with a clinical diagnosis of culture-negative endocarditis, 2, 4, and 2 were positive for\n            <jats:italic>B<\/jats:italic>\n            .\n            <jats:italic>quintana<\/jats:italic>\n            ,\n            <jats:italic>B<\/jats:italic>\n            .\n            <jats:italic>henselae<\/jats:italic>\n            , and\n            <jats:italic>C<\/jats:italic>\n            .\n            <jats:italic>burnetii<\/jats:italic>\n            , respectively, by the dPCR assay, which matched the results obtained by universal bacterial 16S rRNA gene amplification and sequencing.\n          <\/jats:p>","DOI":"10.1128\/jcm.00721-09","type":"journal-article","created":{"date-parts":[[2009,6,25]],"date-time":"2009-06-25T02:13:44Z","timestamp":1245896024000},"page":"2647-2650","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Duplex PCR Assay Simultaneously Detecting and Differentiating\n            <i>Bartonella quintana<\/i>\n            ,\n            <i>B<\/i>\n            .\n            <i>henselae<\/i>\n            , and\n            <i>Coxiella burnetii<\/i>\n            in Surgical Heart Valve Specimens"],"prefix":"10.1128","volume":"47","author":[{"given":"Yi-Wei","family":"Tang","sequence":"first","affiliation":[{"name":"Departments of Pathology and Medicine, Vanderbilt University Medical Center, Nashville, Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/375592"},{"key":"e_1_3_2_3_2","first-page":"1258","volume":"36","year":"1990","unstructured":"Bottger, E. 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