{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T01:13:18Z","timestamp":1792199598379,"version":"4.4.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Rapid diagnosis and treatment of infectious meningitis and encephalitis are critical to minimize morbidity and mortality. Comprehensive testing of cerebrospinal fluid (CSF) often includes Gram stain, culture, antigen detection, and molecular methods, paired with chemical and cellular analyses. These methods may lack sensitivity or specificity, can take several days, and require significant volume for complete analysis. The FilmArray Meningitis\/Encephalitis (ME) Panel is a multiplexed\n                    <jats:italic>in vitro<\/jats:italic>\n                    diagnostic test for the simultaneous, rapid (\u223c1-h) detection of 14 pathogens directly from CSF specimens:\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    K1,\n                    <jats:named-content content-type=\"genus-species\">Haemophilus influenzae<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Streptococcus agalactiae<\/jats:named-content>\n                    , cytomegalovirus, enterovirus, herpes simplex virus 1 and 2, human herpesvirus 6, human parechovirus, varicella-zoster virus, and\n                    <jats:named-content content-type=\"genus-species\">Cryptococcus neoformans<\/jats:named-content>\n                    \/\n                    <jats:named-content content-type=\"genus-species\">Cryptococcus gattii<\/jats:named-content>\n                    . We describe a multicenter evaluation of 1,560 prospectively collected CSF specimens with performance compared to culture (bacterial analytes) and PCR (all other analytes). The FilmArray ME Panel demonstrated a sensitivity or positive percentage of agreement of 100% for 9 of 14 analytes. Enterovirus and human herpesvirus type 6 had agreements of 95.7% and 85.7%, and\n                    <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">N. meningitidis<\/jats:named-content>\n                    were not observed in the study. For\n                    <jats:named-content content-type=\"genus-species\">S. agalactiae<\/jats:named-content>\n                    , there was a single false-positive and false-negative result each, for a sensitivity and specificity of 0 and 99.9%, respectively. The specificity or negative percentage of agreement was 99.2% or greater for all other analytes. The FilmArray ME Panel is a sensitive and specific test to aid in diagnosis of ME. With use of this comprehensive and rapid test, improved patient outcomes and antimicrobial stewardship are anticipated.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00730-16","type":"journal-article","created":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T01:34:34Z","timestamp":1466732074000},"page":"2251-2261","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":562,"title":["Multicenter Evaluation of BioFire FilmArray Meningitis\/Encephalitis Panel for Detection of Bacteria, Viruses, and Yeast in Cerebrospinal Fluid Specimens"],"prefix":"10.1128","volume":"54","author":[{"given":"Amy L.","family":"Leber","sequence":"first","affiliation":[{"name":"Nationwide Children's Hospital, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy","family":"Everhart","sequence":"additional","affiliation":[{"name":"Nationwide Children's Hospital, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan-Miquel","family":"Balada-Llasat","sequence":"additional","affiliation":[{"name":"The Ohio State University Wexner Medical Center, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jillian","family":"Cullison","sequence":"additional","affiliation":[{"name":"The Ohio State University Wexner Medical Center, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Daly","sequence":"additional","affiliation":[{"name":"Primary Children's Medical Center, Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Holt","sequence":"additional","affiliation":[{"name":"Primary Children's Medical Center, Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Lephart","sequence":"additional","affiliation":[{"name":"Detroit Medical Center, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Salimnia","sequence":"additional","affiliation":[{"name":"Detroit Medical Center, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul C.","family":"Schreckenberger","sequence":"additional","affiliation":[{"name":"Loyola University Medical Center, Maywood, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon","family":"DesJarlais","sequence":"additional","affiliation":[{"name":"Loyola University Medical Center, Maywood, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.","family":"Reed","sequence":"additional","affiliation":[{"name":"University of California San Diego School of Medicine, San Diego, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberle C.","family":"Chapin","sequence":"additional","affiliation":[{"name":"Rhode Island Hospital, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay","family":"LeBlanc","sequence":"additional","affiliation":[{"name":"Rhode Island Hospital, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Kristie","family":"Johnson","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole L.","family":"Soliven","sequence":"additional","affiliation":[{"name":"University of Maryland School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen C.","family":"Carroll","sequence":"additional","affiliation":[{"name":"The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo-Anne","family":"Miller","sequence":"additional","affiliation":[{"name":"The Johns Hopkins Hospital, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Dien Bard","sequence":"additional","affiliation":[{"name":"Children's Hospital Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Mestas","sequence":"additional","affiliation":[{"name":"Children's Hospital Los Angeles, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Bankowski","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory Services (The Queen's Medical Center), Aiea, Hawaii, USA"},{"name":"John A. 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