{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:27:12Z","timestamp":1792787232235,"version":"4.5.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"The Open Project Fund of the Key Laboratory of Coal Environmental Pathogenicity and Prevention","award":["MEKLCEPP\/SXMU-202419"],"award-info":[{"award-number":["MEKLCEPP\/SXMU-202419"]}]},{"name":"The Guangxi Natural Science Foundatin","award":["2025GXNSFHA069102"],"award-info":[{"award-number":["2025GXNSFHA069102"]}]},{"name":"Youth Fund for Enhancing Capability of Infectious Disease Surveillance and Prevention","award":["0104748"],"award-info":[{"award-number":["0104748"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2025,11,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The reported human infections with the emerging zoonotic pathogen\n                      <jats:italic toggle=\"yes\">Streptococcus parasuis<\/jats:italic>\n                      are steadily rising. Rapid and standardized genotyping tools specific to\n                      <jats:italic toggle=\"yes\">S. parasuis<\/jats:italic>\n                      are critically needed for epidemiological surveillance and identification of strains with zoonotic potential. This study developed a whole-genome sequence (WGS)-based typing strategy, encompassing average nucleotide identity, a minimum core genome (MCG) typing scheme, and a multilocus sequence typing (MLST) scheme using 255\n                      <jats:italic toggle=\"yes\">S<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">parasuis<\/jats:italic>\n                      genomes isolated from eight countries between the 1980s and 2024. The\n                      <jats:italic toggle=\"yes\">S. parasuis<\/jats:italic>\n                      population was categorized into 12 MCG clusters based on 72,172 SNPs in non-recombining regions distributed across an MCG comprising 607 genes, forming two distinct lineages. The rapid MCG typing program accurately assigned 92.5% of\n                      <jats:italic toggle=\"yes\">S. parasuis<\/jats:italic>\n                      genomes to their corresponding MCG clusters by identifying 4,509 cluster\/subcluster-specific SNPs. To elucidate the clonal relationships among\n                      <jats:italic toggle=\"yes\">S. parasuis<\/jats:italic>\n                      genomes, an MLST scheme was developed, defining 161 sequence types (STs) based on the allelic profiles of seven housekeeping\n                      <jats:italic toggle=\"yes\">loci<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">aroA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">cpn60<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">gki<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">mutS<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">sdhA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">recA<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">thrA<\/jats:italic>\n                      ). Thirty-two STs that shared identical alleles at 6\n                      <jats:italic toggle=\"yes\">loci<\/jats:italic>\n                      were assigned to 10 complex clones, whereas 100 STs that shared identical alleles at 4 or more\n                      <jats:italic toggle=\"yes\">loci<\/jats:italic>\n                      were grouped into 9 ST clades. The MCG typing scheme and the MLST scheme demonstrated sufficient discriminatory power, with Simpson\u2019s diversity index values of 0.8864 and 0.9821, respectively. This study characterized the\n                      <jats:italic toggle=\"yes\">S. parasuis<\/jats:italic>\n                      population and provided a rapid, reproducible, and expandable WGS-based typing strategy for taxonomic identification, epidemiological surveillance, and evaluation of the zoonotic potential of\n                      <jats:italic toggle=\"yes\">S. parasuis<\/jats:italic>\n                      .\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Our study provides valuable insights for developing effective prevention and control strategies for\n                        <jats:italic toggle=\"yes\">Streptococcus parasuis<\/jats:italic>\n                        infections, by revealing the structural characteristics and phylogenetic relationship of\n                        <jats:italic toggle=\"yes\">S. parasuis<\/jats:italic>\n                        population, by developing a whole-genome sequence-based typing strategy applicable for epidemiological surveillance, transmission investigation, and zoonotic potential evaluation.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.00741-25","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T13:26:47Z","timestamp":1761053207000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Developing genome typing strategies for the emerging zoonotic pathogen\n                    <i>Streptococcus parasuis<\/i>"],"prefix":"10.1128","volume":"63","author":[{"given":"Xiyan","family":"Zhang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wktzw65","id-type":"ROR","asserted-by":"publisher"}],"name":"National Institute for Communicable Disease Control and Prevention, Chinese Center for 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