{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:43:40Z","timestamp":1784335420098,"version":"3.55.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Newer \u03b2-lactamases such as extended-spectrum \u03b2-lactamases (ESBLs), transferable AmpC \u03b2-lactamases, and carbapenemases are associated with laboratory testing problems of false susceptibility that can lead to inappropriate therapy for infected patients. Because there appears to be a lack of awareness of these enzymes, a study was conducted during 2001 to 2002 in which 6,421 consecutive, nonduplicate clinical isolates of aerobically growing gram-negative bacilli from patients at 42 intensive care unit (ICU) and 21 non-ICU sites across the United States were tested on-site for antibiotic susceptibility. From these isolates, 746 screen-positive isolates (11.6%) were referred to a research facility and investigated to determine the prevalence of ESBLs in all gram-negative isolates, transferable AmpC \u03b2-lactamases in\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            , and carbapenemases in\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            . The investigations involved phenotypic tests, isoelectric focusing, \u03b2-lactamase inhibitor studies, spectrophotometric assays, induction assays, and molecular analyses. ESBLs were detected only in\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            (4.9% of all\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            ) and were found in species other than those currently recommended for ESBL testing by the CLSI (formerly NCCLS). These isolates occurred at 74% of the ICU sites and 43% of the non-ICU sites. Transferable AmpC \u03b2-lactamases were detected in 3.3% of\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            isolates and at 16 of the 63 sites (25%) with no difference between ICU and non-ICU sites. Three sites submitted isolates that produced class A carbapenemases. No class B or D carbapenemases were detected. In conclusion, organisms producing ESBLs and transferable AmpC \u03b2-lactamases were widespread. Clinical laboratories must be able to detect important \u03b2-lactamases to ensure optimal patient care and infection control.\n          <\/jats:p>","DOI":"10.1128\/jcm.00756-06","type":"journal-article","created":{"date-parts":[[2006,9,5]],"date-time":"2006-09-05T16:23:28Z","timestamp":1157473408000},"page":"3318-3324","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["Prevalence of Newer \u03b2-Lactamases in Gram-Negative Clinical Isolates Collected in the United States from 2001 to 2002"],"prefix":"10.1128","volume":"44","author":[{"given":"Ellen S.","family":"Moland","sequence":"first","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology (CRAB), Department of Medical Microbiology &amp; Immunology, School of Medicine, Creighton University Medical Center, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy D.","family":"Hanson","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology (CRAB), Department of Medical Microbiology &amp; Immunology, School of Medicine, Creighton University Medical Center, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer A.","family":"Black","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology (CRAB), Department of Medical Microbiology &amp; Immunology, School of Medicine, Creighton University Medical Center, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashfaque","family":"Hossain","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology (CRAB), Department of Medical Microbiology &amp; Immunology, School of Medicine, Creighton University Medical Center, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wonkeun","family":"Song","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology (CRAB), Department of Medical Microbiology &amp; Immunology, School of Medicine, Creighton University Medical Center, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth S.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology (CRAB), Department of Medical Microbiology &amp; Immunology, School of Medicine, Creighton University Medical Center, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.2.533-537.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01647010"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3110-3113.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.2.776-778.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki175"},{"key":"e_1_3_2_7_2","unstructured":"Clinical and Laboratory Standards Institute. 2005. 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