{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:50:28Z","timestamp":1790707828328,"version":"4.1.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Clinical outcomes of melioidosis patients improve when the infecting agent,\n            <jats:named-content content-type=\"genus-species\">Burkholderia pseudomallei<\/jats:named-content>\n            , is rapidly detected and identified by laboratory testing. Detection of\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            DNA or recovery of the pathogen by culture from urine can support a diagnosis of melioidosis and guide patient care. Two new methods, designated filter-capture DNA isolation (FCDI) and filter cellular recovery (FCR), were developed to increase the sensitivity of detection and recovery of viable\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            cells from small volumes (0.45 ml) of urine. DNA from eight strains of\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            that were spiked into synthetic urine at low concentrations (1 \u00d7 10\n            <jats:sup>2<\/jats:sup>\n            CFU\/ml) was detected in FCDI cell lysates using real-time PCR with greater consistency than with preparations from a QIAamp DNA Blood minikit. The FCR method showed greater\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            detection sensitivity than conventional urine culture methods and resulted in typical colony growth at 24 h from as few as 1 \u00d7 10\n            <jats:sup>2<\/jats:sup>\n            CFU\/ml. In addition, the FCR method does not rely on precipitation of a urine pellet by centrifugation and requires a smaller volume of urine. The FCDI and FCR methods described here could improve time-to-results and decrease the number of negative\n            <jats:named-content content-type=\"genus-species\">B. pseudomallei<\/jats:named-content>\n            reports that are currently observed from urine culture as a consequence of samples containing low or variable bacterial cell concentrations.\n          <\/jats:p>","DOI":"10.1128\/jcm.00764-17","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T00:40:27Z","timestamp":1498092027000},"page":"2698-2707","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Rapid Filter-Based Detection and Culture of Burkholderia pseudomallei from Small Volumes of Urine"],"prefix":"10.1128","volume":"55","author":[{"given":"Pierre A.","family":"Michel","sequence":"first","affiliation":[{"name":"National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Lascols","sequence":"additional","affiliation":[{"name":"National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay E.","family":"Gee","sequence":"additional","affiliation":[{"name":"National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda M.","family":"Weigel","sequence":"additional","affiliation":[{"name":"National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sue","sequence":"additional","affiliation":[{"name":"National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2004.00797.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13374-0"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.383-416.2005"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.177"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0912.020750"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2015.8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/529290a"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/jtm.12236"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.5005\/jp\/books\/11617_52"},{"key":"e_1_3_3_11_2","first-page":"1","article-title":"Melioidosis cases and selected reports of occupational exposures to Burkholderia pseudomallei\u2014United States, 2008\u20132013","volume":"64","author":"Benoit TJ","year":"2015","unstructured":"Benoit TJ Blaney DD Gee JE Elrod MG Hoffmaster AR Doker TJ Bower WA Walke HT , Centers for Disease C, Prevention. 2015. 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