{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:50:25Z","timestamp":1790707825428,"version":"4.1.0"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Extended-spectrum beta-lactamases (ESBL) of the TEM, SHV, or CTX-M type confer resistance to beta-lactam antibiotics in Gram-negative bacteria. The activity of these enzymes against beta-lactam antibiotics and their resistance against inhibitors can be influenced by genetic variation at the single-nucleotide level. Here, we describe the development and validation of an oligonucleotide microarray for the rapid identification of ESBLs in Gram-negative bacteria by simultaneously genotyping<jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub>, and<jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub>. The array consists of 618 probes that cover mutations responsible for 156 amino acid substitutions. As this comprises unprecedented genotyping coverage, the ESBL array has a high potential for epidemiological studies and infection control. With an assay time of 5 h, the ESBL microarray also could be an attractive option for the development of rapid antimicrobial resistance tests in the future. The validity of the DNA microarray was demonstrated with 60 blinded clinical isolates, which were collected during clinical routines. Fifty-eight of them were characterized phenotypically as ESBL producers. The chip was characterized with regard to its resolution, phenotype-genotype correlation, and ability to resolve mixed genotypes. ESBL phenotypes could be correctly ascribed to ESBL variants of<jats:italic>bla<\/jats:italic><jats:sub>CTX-M<\/jats:sub>(76%),<jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub>(22%), or both (2%), whereas no ESBL variant of<jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>was found. The most prevalent ESBLs identified were CTX-M-15 (57%) and SHV-12 (18%).<\/jats:p>","DOI":"10.1128\/jcm.00765-09","type":"journal-article","created":{"date-parts":[[2009,12,10]],"date-time":"2009-12-10T02:40:46Z","timestamp":1260412846000},"page":"460-471","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Integrated Detection of Extended-Spectrum-Beta-Lactam Resistance by DNA Microarray-Based Genotyping of TEM, SHV, and CTX-M Genes"],"prefix":"10.1128","volume":"48","author":[{"given":"Dirk M.","family":"Leinberger","sequence":"first","affiliation":[{"name":"Division of Pathway Medicine, University of Edinburgh, Edinburgh, United Kingdom"},{"name":"Institute of Technical Biochemistry, University of Stuttgart, Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Verena","family":"Grimm","sequence":"additional","affiliation":[{"name":"Institute of Technical Biochemistry, University of Stuttgart, Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Rubtsova","sequence":"additional","affiliation":[{"name":"Chemistry Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Weile","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine, Robert Bosch Hospital, Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Schro\u0308ppel","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, Eberhard-Karls-University, Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Wichelhaus","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Infection Control, J. W. Goethe-Universita\u0308t, Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelius","family":"Knabbe","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine, Robert Bosch Hospital, Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf D.","family":"Schmid","sequence":"additional","affiliation":[{"name":"Institute of Technical Biochemistry, University of Stuttgart, Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Till T.","family":"Bachmann","sequence":"additional","affiliation":[{"name":"Division of Pathway Medicine, University of Edinburgh, Edinburgh, United Kingdom"},{"name":"Institute of Technical Biochemistry, University of Stuttgart, Stuttgart, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2760269"},{"key":"e_1_3_2_3_2","first-page":"203","volume":"134","year":"1995","unstructured":"Arlet, G., G. Brami, D. Decre, A. Flippo, O. Gaillot, P. H. Lagrange, and A. Philippon. 1995. Molecular characterization by PCR-restriction fragment length polymorphism of TEM beta-lactamases. FEMS Microbiol. Lett. 134 : 203-208.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2007.11.017"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46909-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.5.3780-3784.2006"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2006.08.011"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01849.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01850.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01574-05"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2004.11.008"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02291-05"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","unstructured":"Performance standards for antimicrobial susceptibility testing 2009 vol. 29","DOI":"10.1592\/phco.29.11.1326"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2006.07.008"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01846.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki398"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2005.09.021"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01854.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3766-3774.2004"},{"key":"e_1_3_2_21_2","first-page":"95","volume":"41","year":"1999","unstructured":"Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT. Nucl. Acids Symp. Ser. 41 : 95-98.","journal-title":"Nucl. Acids Symp. Ser."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.3045-3049.2002"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01155-08"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.5.1520-1525.2004"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.28.2.302"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/S1016-8478(23)15092-8","volume":"14","year":"2002","unstructured":"Lee, Y., C. S. Lee, Y. J. Kim, S. Chun, S. Park, Y. S. Kim, and B. D. Han. 2002. Development of DNA chip for the simultaneous detection of various beta-lactam antibiotic-resistant genes. Mol. Cells 14 : 192-197.","journal-title":"Mol. Cells"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.10.4943-4953.2005"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl483"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01871.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2007.04.007"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01243.x"},{"key":"e_1_3_2_32_2","unstructured":"J. Antimicrob. Chemother 1998"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00611-06"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.2.616-620.2001"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.5.2112-2115.2005"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.657-686.2005"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.6.2206-2212.2001"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.4038-4040.2002"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.6.1730-1736.2001"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4895-4897.2004"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01867.x"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm318"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.4.1257-1262.2006"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4891-4894.2005"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.879"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2006.10.003"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.04.026"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-2779-8"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.06.005"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01988-06"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1086\/324742"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki412"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.9.4083-4091.2004"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01461-06"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00765-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00765-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T04:16:53Z","timestamp":1710735413000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00765-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1128\/JCM.00765-09"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00765-09","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2]]},"assertion":[{"value":"2009-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-11-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}