{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T08:46:44Z","timestamp":1791017204967,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:00:00Z","timestamp":1225497600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            isolates collected from sites of intramammary infection during a 10-month period and from extramammary sites (dairy cow teat skin, teat canals, and skin lesions; milking liners; and hands and nostrils of milking personnel) at two separately managed Finnish dairy herd establishments were analyzed to study the sources and reservoirs of bovine\n            <jats:italic>S. aureus<\/jats:italic>\n            intramammary infection. Selected isolates were subjected to pulsed-field gel electrophoresis (PFGE) typing and PCR analysis for genes encoding hemolysins (\n            <jats:italic>hla<\/jats:italic>\n            to\n            <jats:italic>hlg<\/jats:italic>\n            ), leukocidins (\n            <jats:italic>lukED<\/jats:italic>\n            and\n            <jats:italic>lukM<\/jats:italic>\n            ), superantigens (\n            <jats:italic>sea<\/jats:italic>\n            ,\n            <jats:italic>sec<\/jats:italic>\n            ,\n            <jats:italic>sed<\/jats:italic>\n            ,\n            <jats:italic>seg<\/jats:italic>\n            to\n            <jats:italic>seo<\/jats:italic>\n            ,\n            <jats:italic>seu<\/jats:italic>\n            , and\n            <jats:italic>tst<\/jats:italic>\n            ), adhesins (\n            <jats:italic>fnbA<\/jats:italic>\n            and\n            <jats:italic>fnbB<\/jats:italic>\n            ), and penicillin and methicillin resistance (\n            <jats:italic>blaZ<\/jats:italic>\n            and\n            <jats:italic>mecA<\/jats:italic>\n            ).\n            <jats:italic>S. aureus<\/jats:italic>\n            was found throughout the herds in 94% of the cows. Nine PFGE types were found, with the herds each having their own predominant type and sharing one type. The degree of diversity of PFGE types in herd II, which integrated foreign heifers, was higher than that in herd I. For both herds, the majority of the PFGE-typed isolates both from milk and from extramammary sites represented the predominant PFGE types. In isolates from herd I, the most prevalent genes were\n            <jats:italic>hla<\/jats:italic>\n            -\n            <jats:italic>hlg<\/jats:italic>\n            ,\n            <jats:italic>lukED<\/jats:italic>\n            , and\n            <jats:italic>fnbA<\/jats:italic>\n            ; in those from herd II, they were\n            <jats:italic>hla<\/jats:italic>\n            ,\n            <jats:italic>hld<\/jats:italic>\n            ,\n            <jats:italic>hlg<\/jats:italic>\n            ,\n            <jats:italic>lukED<\/jats:italic>\n            , and\n            <jats:italic>fnbA<\/jats:italic>\n            . The other genes were pulsotype linked within the herds. The predominant PFGE types carried both\n            <jats:italic>fnbA<\/jats:italic>\n            and\n            <jats:italic>fnbB<\/jats:italic>\n            ; only\n            <jats:italic>fnbA<\/jats:italic>\n            was detected in the other PFGE types. No connection between specific virulence genes and the origins of isolates was found. The results suggest that for the two herds, most\n            <jats:italic>S. aureus<\/jats:italic>\n            isolates from extramammary sites were indistinguishable from the isolates infecting the mammary gland and that those sites can thus act as origins and reservoirs of intramammary infections. However, contamination in the opposite direction cannot be excluded.\n          <\/jats:p>","DOI":"10.1128\/jcm.00769-08","type":"journal-article","created":{"date-parts":[[2008,9,18]],"date-time":"2008-09-18T00:55:24Z","timestamp":1221699324000},"page":"3728-3735","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["Molecular Types and Genetic Profiles of\n            <i>Staphylococcus aureus<\/i>\n            Strains Isolated from Bovine Intramammary Infections and Extramammary Sites"],"prefix":"10.1128","volume":"46","author":[{"given":"M.","family":"Haveri","sequence":"first","affiliation":[{"name":"Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hovinen","sequence":"additional","affiliation":[{"name":"Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Roslo\u0308f","sequence":"additional","affiliation":[{"name":"Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Pyo\u0308ra\u0308la\u0308","sequence":"additional","affiliation":[{"name":"Department of Production Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1999.tb01576.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01984-07"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.7.1654-1660.1992"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.2292-2295.2003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4673-4678.1999"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1067\/mlc.2000.105179"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(01)02228-4"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.02.005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2008.01.010"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1278-1284.2005"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_17061143.x"},{"key":"e_1_3_2_13_2","first-page":"97102","volume":"106","year":"2005","unstructured":"Haveri, M., S. 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