{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:25:04Z","timestamp":1791156304990,"version":"4.1.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2006,9,1]],"date-time":"2006-09-01T00:00:00Z","timestamp":1157068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A single-round PCR assay was developed for detection and differential diagnosis of the three\n                    <jats:italic>Entamoeba<\/jats:italic>\n                    species found in humans,\n                    <jats:italic>Entamoeba moshkovskii<\/jats:italic>\n                    ,\n                    <jats:italic>Entamoeba histolytica<\/jats:italic>\n                    , and\n                    <jats:italic>Entamoeba dispar<\/jats:italic>\n                    , that are morphologically identical as both cysts and trophozoites. A conserved forward primer was derived from the middle of the small-subunit rRNA gene, and reverse primers were designed from signature sequences specific to each of these three\n                    <jats:italic>Entamoeba<\/jats:italic>\n                    species. PCR generates a 166-bp product with\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    DNA, a 752-bp product with\n                    <jats:italic>E. dispar<\/jats:italic>\n                    DNA, and a 580-bp product with\n                    <jats:italic>E. moshkovskii<\/jats:italic>\n                    DNA. Thirty clinical specimens were examined, and the species present were successfully detected and differentiated using this assay. It was possible to detect as little as 10 pg of\n                    <jats:italic>E. moshkovskii<\/jats:italic>\n                    and\n                    <jats:italic>E. histolytica<\/jats:italic>\n                    DNA, while for\n                    <jats:italic>E. dispar<\/jats:italic>\n                    the sensitivity was about 20 pg of DNA. Testing with DNA from different pathogens, including bacteria and other protozoa, confirmed the high specificity of the assay. We propose the use of this PCR assay as an accurate, rapid, and effective diagnostic method for the detection and discrimination of these three morphologically indistinguishable\n                    <jats:italic>Entamoeba<\/jats:italic>\n                    species in both routine diagnosis of amoebiasis and epidemiological surveys.\n                  <\/jats:p>","DOI":"10.1128\/jcm.00778-06","type":"journal-article","created":{"date-parts":[[2006,9,5]],"date-time":"2006-09-05T12:23:28Z","timestamp":1157459008000},"page":"3196-3200","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":132,"title":["Differential Detection of\n                    <i>Entamoeba histolytica<\/i>\n                    ,\n                    <i>Entamoeba dispar<\/i>\n                    , and\n                    <i>Entamoeba moshkovskii<\/i>\n                    by a Single-Round PCR Assay"],"prefix":"10.1128","volume":"44","author":[{"given":"Zulhainan","family":"Hamzah","sequence":"first","affiliation":[{"name":"Department of Protozoology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songsak","family":"Petmitr","sequence":"additional","affiliation":[{"name":"Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathirut","family":"Mungthin","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saovanee","family":"Leelayoova","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Porntip","family":"Chavalitshewinkoon-Petmitr","sequence":"additional","affiliation":[{"name":"Department of Protozoology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"58","volume":"48","year":"1994","unstructured":"Acuna-Soto, R., J. Samuelson, P. De-Girolami, et al. 1994. Application of the polymerase chain reaction to the epidemiology of pathogenic and nonpathogenic Entamoeba histolytica. Am. J. Trop. Med. Hyg.48:58-70.","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0905.020548"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90194-B"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.3.236"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.2.449-452.1998"},{"key":"e_1_3_2_7_2","first-page":"201","volume":"47","year":"1998","unstructured":"Haque, R., I. K. M. Ali, C. G. Clark, and W. A. Petri, Jr. 1998. A case report of Entamoeba moshkovskii infection in a Bangladeshi child. Parasitol. Int.47:201-202.","journal-title":"Parasitol. Int."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.1.247"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.10.2558-2561.1995"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.1031"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.9.2405-2407.1997"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/313433"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.4.825"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.2.250-255.1991"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.5041-5045.2003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1644-1646.2001"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00778-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00778-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:33:03Z","timestamp":1646440383000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00778-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":15,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.1128\/JCM.00778-06"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00778-06","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9]]},"assertion":[{"value":"2006-04-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-07-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}