{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:18:57Z","timestamp":1791091137820,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Louse-borne diseases are prevalent in the homeless, and body louse eradication has thus far been unsuccessful in this population. We aim to develop a rapid and robust genotyping method usable in large field-based clinical studies to monitor permethrin resistance in the human body louse\n            <jats:named-content content-type=\"genus-species\">Pediculus humanus corporis<\/jats:named-content>\n            . We assessed a melting curve analysis genotyping method based on real-time PCR using hybridization probes to detect the M815I-T917I-L920F knockdown resistance (kdr) mutation in the paraorthologous voltage-sensitive sodium channel (VSSC) \u03b1 subunit gene, which is associated with permethrin resistance. The 908-bp DNA fragment of the VSSC gene, encoding the \u03b1 subunit of the sodium channel and encompassing the three mutation sites, was PCR sequenced from 65 lice collected from a homeless population. We noted a high prevalence of the 3 indicated mutations in the body lice collected from homeless people (100% for the M815I and L920F mutations and 56.73% for the T917I mutation). These results were confirmed by melting curve analysis genotyping, which had a calculated sensitivity of 100% for the M815I and T917I mutations and of 98% for the L920F mutation. The specificity was 100% for M815I and L920F and 96% for T917I. Melting curve analysis genotyping is a fast, sensitive, and specific tool that is fully compatible with the analysis of a large number of samples in epidemiological surveys, allowing the simultaneous genotyping of 96 samples in just over an hour (75 min). Thus, it is perfectly suited for the epidemiological monitoring of permethrin resistance in human body lice in large-scale clinical studies.\n          <\/jats:p>","DOI":"10.1128\/jcm.00808-12","type":"journal-article","created":{"date-parts":[[2012,5,10]],"date-time":"2012-05-10T04:59:04Z","timestamp":1336625944000},"page":"2229-2233","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Detection of a Knockdown Resistance Mutation Associated with Permethrin Resistance in the Body Louse Pediculus humanus corporis by Use of Melting Curve Analysis Genotyping"],"prefix":"10.1128","volume":"50","author":[{"given":"Rezak","family":"Drali","sequence":"first","affiliation":[{"name":"Aix-Marseille Universit\u00e9, URMITE CNRS-IRD, UMR 6236\/198 IHU M\u00e9diterran\u00e9e Infection, Facult\u00e9 de M\u00e9decine et de Pharmacie, Marseille, France"},{"name":"Service des Ent\u00e9robact\u00e9ries et Hygi\u00e8ne de l'Environnement, Institut Pasteur d'Alg\u00e9rie, Algiers, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir","family":"Benkouiten","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, URMITE CNRS-IRD, UMR 6236\/198 IHU M\u00e9diterran\u00e9e Infection, Facult\u00e9 de M\u00e9decine et de Pharmacie, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9k\u00e9n\u00e9","family":"Badiaga","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, URMITE CNRS-IRD, UMR 6236\/198 IHU M\u00e9diterran\u00e9e Infection, Facult\u00e9 de M\u00e9decine et de Pharmacie, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idir","family":"Bitam","sequence":"additional","affiliation":[{"name":"Service d'Ecologie des Syst\u00e8mes Vectoriels, IRD 198, Institut Pasteur d'Alg\u00e9rie, Algiers, Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Marc","family":"Rolain","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, URMITE CNRS-IRD, UMR 6236\/198 IHU M\u00e9diterran\u00e9e Infection, Facult\u00e9 de M\u00e9decine et de Pharmacie, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Brouqui","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9, URMITE CNRS-IRD, UMR 6236\/198 IHU M\u00e9diterran\u00e9e Infection, Facult\u00e9 de M\u00e9decine et de Pharmacie, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn161"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-6-111"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.6.1513"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.md.0000152373.07500.6e"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)09458-1"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aspen.2008.10.007"},{"key":"e_1_3_3_8_2","first-page":"63","article-title":"Permethrin resistance due to knockdown gene mutations is prevalent in human head louse populations","volume":"4","author":"Clark JM","year":"2010","unstructured":"ClarkJM. 2010. 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