{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T21:03:03Z","timestamp":1784408583536,"version":"3.55.0"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["23fk0108676,24fk0108676"],"award-info":[{"award-number":["23fk0108676,24fk0108676"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2024,12,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The promising diagnostic performance of molecular testing for syphilis using saliva and urine samples has been reported; however, further evaluation of its possible application for diagnosis and molecular surveillance is required. In addition, the development of a rapid and easy-to-perform molecular test for syphilis is important for its use in the clinical setting. We comprehensively evaluated the diagnostic and surveillance performance of two novel loop-mediated isothermal amplification (LAMP) assays using saliva and urine samples. Saliva, urine, and whole blood were collected from patients who underwent serological testing for syphilis at outpatient clinics.\n              <jats:italic>Treponema pallidum<\/jats:italic>\n              DNA in specimens was detected using quantitative PCR (qPCR), nested PCR, and novel LAMP assays.\n              <jats:italic>T. pallidum<\/jats:italic>\n              genotyping was conducted by multi-locus sequence typing (MLST). Of the 163 patients recruited, 98 were diagnosed with syphilis (primary:\n              <jats:italic>n<\/jats:italic>\n              = 35; secondary:\n              <jats:italic>n<\/jats:italic>\n              = 40; latent:\n              <jats:italic>n<\/jats:italic>\n              = 23). qPCR showed the highest sensitivity among the molecular tests performed with a sensitivity of 54.1% and 30.3% for all syphilis patients using saliva and urine samples, respectively. A novel method of LAMP combined with dry reagents and crude DNA extraction (Dry-LAMP) showed a probit detection limit of 37.4 copies\/reaction within 45 min. The agreement rate between Dry-LAMP and qPCR for saliva was 95.7% (\n              <jats:italic>\u03ba<\/jats:italic>\n              coefficient 0.90). The\n              <jats:italic>T. pallidum<\/jats:italic>\n              genotype was identified in 48 patients by MLST using saliva samples. Molecular analysis of saliva could be used as a supplementary diagnostic test for syphilis and molecular surveillance of the\n              <jats:italic>T. pallidum<\/jats:italic>\n              genotype. Dry-LAMP is expected to be helpful in the clinical diagnosis of syphilis.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/jcm.00809-24","type":"journal-article","created":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T14:00:58Z","timestamp":1730901658000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Prospective evaluation of non-invasive saliva specimens for the diagnosis of syphilis and molecular surveillance of\n            <i>Treponema pallidum<\/i>"],"prefix":"10.1128","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8416-7371","authenticated-orcid":true,"given":"Kazuo","family":"Imai","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Saitama Medical University Hospital","place":["Saitama, Japan"]},{"name":"Department of Infectious Disease and Infection Control, Saitama Medical University","place":["Saitama, Japan"]},{"name":"KARADA Internal Medicine Clinic","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Sato","sequence":"additional","affiliation":[{"name":"KARADA Internal Medicine Clinic","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Tanaka","sequence":"additional","affiliation":[{"name":"KARADA Internal Medicine Clinic Shibuya","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Ohama","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I, National Institute of Infectious Diseases","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1495-0926","authenticated-orcid":false,"given":"Shu-ichi","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I, National Institute of Infectious Diseases","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuha","family":"Omachi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Saitama Medical University Hospital","place":["Saitama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Saitama Medical University Hospital","place":["Saitama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5866-8877","authenticated-orcid":false,"given":"Norihito","family":"Tarumoto","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Infection Control, Saitama Medical University","place":["Saitama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mieko","family":"Tokano","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Infection Control, Saitama Medical University","place":["Saitama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigefumi","family":"Mesaki","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease and Infection Control, Saitama Medical University","place":["Saitama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0912-4643","authenticated-orcid":false,"given":"Takuya","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Saitama Medical University Hospital","place":["Saitama, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5325-4173","authenticated-orcid":false,"given":"Yukihiro","family":"Akeda","sequence":"additional","affiliation":[{"name":"Department of Bacteriology I, National Institute of Infectious Diseases","place":["Tokyo, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.18.228486"},{"key":"e_1_3_5_3_2","unstructured":"World Health Organization. 2024. Syphilis-overview. Available from: https:\/\/www.who.int\/health-topics\/syphilis#tab=tab_1. Retrieved 28 May 2024."},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181e1a77a"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2011.049494"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2015.04.018"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiad582"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1613"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01772-21"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000956"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_3_5_12_2","unstructured":"World Health Organization. Who evidence review group on malaria diagnosis in low transmission settings. Available from: https:\/\/www.who.int\/docs\/default-source\/malaria\/mpac-documentation\/mpac-mar2014-diagnosis-low-transmission-settings-report.pdf?sfvrsn=5694f918_2. Retrieved 2828 MayMay 2024. Accessed 2828 MayMay 2024"},{"key":"e_1_3_5_13_2","unstructured":"World Health Organization. 2024. The use of loop-mediated isothermal amplification (tb-lamp) for the diagnosis of pulmonary tuberculosis: policy guidance. Available from: https:\/\/apps.who.int\/iris\/bitstream\/handle\/10665\/249154\/9789241511186-eng.pdf?sequence=1&isAllowed=y(accessed. Retrieved 28 May 2024."},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0051"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-022-02973-z"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2602.190505"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2021-058605"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006396"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b05371"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/656533"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0200773"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010063"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000001378"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2008.034314"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00720-09"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41426-018-0085-2"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.5.1941-1946.2001"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2508.181690"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msw054"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127077"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2021.11.012"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00070-13"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009900"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2018.02.006"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00809-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jcm.00809-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T19:08:38Z","timestamp":1733944118000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jcm.00809-24"}},"subtitle":[],"editor":[{"given":"Daniel J.","family":"Diekema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,12,11]]},"references-count":33,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12,11]]}},"alternative-id":["10.1128\/jcm.00809-24"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00809-24","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,11]]},"assertion":[{"value":"2024-06-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00809-24"}}