{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:26:28Z","timestamp":1791152788456,"version":"4.1.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2008,8,1]],"date-time":"2008-08-01T00:00:00Z","timestamp":1217548800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Previous epidemiological assessments of the prevalence versus special-pathogenicity hypothesis for urinary tract infection (UTI) pathogenesis in women may have been confounded by underlying host population differences between women with UTI and healthy controls and have not considered the clonal complexity of the fecal\n            <jats:italic>Escherichia coli<\/jats:italic>\n            population of the host. In the present study, 42 women with acute uncomplicated cystitis served as their own controls for an analysis of the causative\n            <jats:italic>E. coli<\/jats:italic>\n            strain and the concurrent intestinal\n            <jats:italic>E. coli<\/jats:italic>\n            population. Clonality among the urine isolate and 30 fecal colonies per subject was assessed by repetitive-element PCR and macrorestriction analysis. Each unique clone underwent PCR-based phylotyping and virulence genotyping. Molecular analysis resolved 109 unique clones (4 urine-only, 38 urine-fecal, and 67 fecal-only clones). Urine clones exhibited a significantly higher prevalence of group B2 than fecal-only clones (69% versus 10%;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001) and higher aggregate virulence scores (mean, 6.2 versus 2.9;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). In multilevel regression models for predicting urine clone status, significant positive predictors included group B2, 10 individual virulence traits, the aggregate virulence score, fecal dominance, relative fecal abundance, and (unique to the present study) a pauciclonal fecal sample. In summary, within the fecal\n            <jats:italic>E. coli<\/jats:italic>\n            populations of women with acute cystitis, pauciclonality, clonal dominance, virulence, and group B2 status are closely intertwined. Phylogenetic group B2 status and\/or associated virulence factors may promote fecal abundance and pauciclonality, thereby contributing to upstream steps in UTI pathogenesis. This relationship suggests a possible reconciliation of the prevalence and special-pathogenicity hypotheses.\n          <\/jats:p>","DOI":"10.1128\/jcm.00813-08","type":"journal-article","created":{"date-parts":[[2008,5,22]],"date-time":"2008-05-22T01:22:49Z","timestamp":1211419369000},"page":"2529-2534","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":167,"title":["Relationship between\n            <i>Escherichia coli<\/i>\n            Strains Causing Acute Cystitis in Women and the Fecal\n            <i>E. coli<\/i>\n            Population of the Host"],"prefix":"10.1128","volume":"46","author":[{"given":"Eva","family":"Moreno","sequence":"first","affiliation":[{"name":"Departments of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Andreu","sequence":"additional","affiliation":[{"name":"Departments of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carles","family":"Pigrau","sequence":"additional","affiliation":[{"name":"Infectious Diseases, Hospital Vall d'Hebron, Universitat Auto\u0300noma de Barcelona, Passeig Vall d'Hebron 119-129, 08035 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Kuskowski","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center and University of Minnesota, Minneapolis, Minnesota 55417"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center and University of Minnesota, Minneapolis, Minnesota 55417"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillem","family":"Prats","sequence":"additional","affiliation":[{"name":"Departments of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"15","volume":"23","year":"2005","unstructured":"Andreu, A. 2005. Pathogenesis of urinary tract infections. Enferm. Infecc. Microbiol. Clin.23(Suppl. 4):15-21. (In Spanish.)","journal-title":"Enferm. Infecc. Microbiol. Clin."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.11.2977-2980.1997"},{"key":"e_1_3_2_5_2","first-page":"766","year":"1969","unstructured":"Gruneberg, R. N. 1969. Relationship of infecting urinary organism to the faecal flora in patients with symptomatic urinary infection. 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