{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:10:35Z","timestamp":1788959435172,"version":"build-2803163510"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2026,9,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Accurate drug susceptibility testing (DST) is crucial for designing effective regimens for multidrug-resistant (MDR) and pre-extensively drug-resistant tuberculosis (pre-XDR TB). Sensititre MYCOTB enables simultaneous determination of minimum inhibitory concentrations (MICs) for multiple drugs, but its diagnostic performance varies across studies. This meta-analysis evaluated the diagnostic performance of Sensititre MYCOTB for key MDR and pre-XDR TB drugs. The protocol was registered in PROSPERO (CRD420251230599). PubMed, Cochrane, Google Scholar, Scopus, ONOS, Web of Science, ScienceDirect, and registries were systematically searched for studies published between 2010 and 2025. Studies comparing the Sensititre MYCOTB with reference DST for\n                      <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                      complex (MTBC) were included. Bias assessment and pooled diagnostic accuracy estimates were generated. Fourteen studies, including 1,728 isolates, were analyzed. Rifampicin and isoniazid demonstrated high sensitivity (0.976 [95% CI: 0.94\u20130.99] and 0.977 [95% CI: 0.95\u20130.99]) and specificity (0.958 [95% CI: 0.84\u20130.98] and 0.957 [95% CI: 0.83\u20130.99], respectively) with low heterogeneity. Amikacin, kanamycin, and ofloxacin demonstrate good diagnostic accuracy, with high specificity (&gt;0.98 [95% CI]). Moderate diagnostic accuracy was observed for ethambutol, streptomycin, ethionamide, and rifabutin. Cycloserine, moxifloxacin, and para-aminosalicylic acid showed inconsistent performance despite excellent specificity (&gt;0.97 [95% CI]). Sensitivity analysis partially improved pooled sensitivity for moxifloxacin 0.801 (95% CI: 0.585\u20130.924) and para-aminosalicylic acid 0.76 (95% CI: 0.518\u20130.894), whereas cycloserine remained at 0.436 (95% CI: 0.190\u20130.725), although heterogeneity persisted. Sensititre MYCOTB DST demonstrates high diagnostic accuracy for MDR-TB and pre-XDR-TB drugs, while caution is required with cycloserine, moxifloxacin, and para-aminosalicylic acid. These findings support the integration of MIC-based testing into clinical decision-making.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jcm.00813-26","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T17:51:25Z","timestamp":1787248285000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["MIC-based tuberculosis drug susceptibility testing using Sensititre MYCOTB: a diagnostic accuracy meta-analysis"],"prefix":"10.1128","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8187-3840","authenticated-orcid":false,"given":"Daniel Raj","family":"D","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rs0zz87","id-type":"ROR","asserted-by":"publisher"}],"name":"Medical Laboratory Technology (Microbiology), AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukesh Kumar","family":"G","sequence":"additional","affiliation":[{"name":"Psychiatric Nursing, AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5626-6076","authenticated-orcid":true,"given":"Jitendra","family":"Singh","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rs0zz87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Translational Medicine, AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabir","family":"Ali","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rs0zz87","id-type":"ROR","asserted-by":"publisher"}],"name":"Medical Research Unit (MRU), AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaina","family":"Gaikwad","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rs0zz87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tharani","family":"Mohankumar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rs0zz87","id-type":"ROR","asserted-by":"publisher"}],"name":"Medical Laboratory Technology (Microbiology), AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swathi","family":"S","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rs0zz87","id-type":"ROR","asserted-by":"publisher"}],"name":"Medical Laboratory Technology (Microbiology), AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shashank","family":"Purwar","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rs0zz87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debasis","family":"Biswas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rs0zz87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4827-919X","authenticated-orcid":true,"given":"Anand Kumar","family":"Maurya","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rs0zz87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, AIIMS","place":["Bhopal, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjit","family":"Sah","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Dr D.Y. 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