{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:18:22Z","timestamp":1761488302708},"reference-count":24,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            With the support of a ministerial program for innovative and expensive technologies, dedicated to the economic evaluation of laboratory diagnosis of pertussis by real-time PCR, external quality assessment for real-time IS\n            <jats:italic>481<\/jats:italic>\n            PCR was carried out. Coordinated by the National Centre of Reference of Pertussis and other Bordetelloses (NCR), this study aimed to harmonize and to assess the performances of eight participating microbiology hospital laboratories throughout the French territory. Between January 2006 and February 2007, 10 proficiency panels were sent by the NCR (ascending proficiency program), representing a total of 49 samples and including eight panels to analyze and evaluate the global sensitivity and specificity of real-time PCR, one to assess the limit of detection, and one to evaluate nucleic acid extraction methods. As part of the descending proficiency program, extracted DNA from clinical samples was sent by the eight participating laboratories in different panels and analyzed by the NCR. In the ascending proficiency analysis, the sensitivity and specificity of the real-time PCR methods were 92.2% and 94.3%, respectively. The limit of detection of the different methods ranged between 0.1 and 1 fg\/\u03bcl (0.2 to 2 CFU\/\u03bcl). The nucleic acid extraction methods showed similar performances. During the descending proficiency analysis, performed with 126 samples, the result of the NCR for 15 samples (11.9%) was discordant with the result obtained by the source laboratory. Despite several initial differences, harmonization was easy and performances were homogeneous. However, the risk of false-positive results remains quite high, and we strongly recommend establishment of uniform quality control procedures performed regularly.\n          <\/jats:p>","DOI":"10.1128\/jcm.00817-09","type":"journal-article","created":{"date-parts":[[2009,8,20]],"date-time":"2009-08-20T01:50:48Z","timestamp":1250733048000},"page":"3197-3203","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Proficiency Program for Real-Time PCR Diagnosis of\n            <i>Bordetella pertussis<\/i>\n            Infections in French Hospital Laboratories and at the French National Reference Center for Whooping Cough and other Bordetelloses"],"prefix":"10.1128","volume":"47","author":[{"given":"Vale\u0301rie","family":"Caro","sequence":"first","affiliation":[{"name":"Institut Pasteur, Centre National de Re\u0301fe\u0301rence (CNR) de la Coqueluche et autres Bordetelloses, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Guiso","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Centre National de Re\u0301fe\u0301rence (CNR) de la Coqueluche et autres Bordetelloses, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinne","family":"Alberti","sequence":"additional","affiliation":[{"name":"Unite\u0301 d'Epide\u0301miologie Clinique, Ho\u0302pital Robert-Debre\u0301, APHP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandrine","family":"Liguori","sequence":"additional","affiliation":[{"name":"Services de Microbiologie, Ho\u0302pital Robert-Debre\u0301, APHP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Burucoa","sequence":"additional","affiliation":[{"name":"CHU La Mile\u0301trie, Poitiers, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ge\u0301rard","family":"Couetdic","sequence":"additional","affiliation":[{"name":"CHU Jean Minjoz, Besanc\u0327on, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florence","family":"Doucet-Populaire","sequence":"additional","affiliation":[{"name":"C.H. de Versailles, Le Chesnay, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agne\u0300s","family":"Ferroni","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Necker-enfants malades, APHP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Papin-Gibaud","sequence":"additional","affiliation":[{"name":"CHU Nantes, Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florence","family":"Grattard","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Nord, Saint Etienne, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He\u0301le\u0300ne","family":"Re\u0301glier-Poupet","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Cochin Saint Vincent de Paul, APHP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josette","family":"Raymond","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Cochin Saint Vincent de Paul, APHP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Soler","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Nord, Saint Etienne, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Bouchet","sequence":"additional","affiliation":[{"name":"CHU Nantes, Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandrine","family":"Charreau","sequence":"additional","affiliation":[{"name":"CHU La Mile\u0301trie, Poitiers, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Couzon","sequence":"additional","affiliation":[{"name":"C.H. de Versailles, Le Chesnay, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Leymarie","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Necker-enfants malades, APHP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Tavares","sequence":"additional","affiliation":[{"name":"Ho\u0302pital Cochin Saint Vincent de Paul, APHP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathilde","family":"Choux","sequence":"additional","affiliation":[{"name":"CHU Jean Minjoz, Besanc\u0327on, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edouard","family":"Bingen","sequence":"additional","affiliation":[{"name":"Services de Microbiologie, Ho\u0302pital Robert-Debre\u0301, APHP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ste\u0301phane","family":"Bonacorsi","sequence":"additional","affiliation":[{"name":"Services de Microbiologie, Ho\u0302pital Robert-Debre\u0301, APHP, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02187-07"},{"key":"e_1_3_2_3_2","first-page":"147","volume":"16","year":"2009","unstructured":"Anonymous. 2009. Calendrier vaccinal 2009\u2014Avis du Haut conseil de la sant\u00e9 publique. Bull. Epidemiol. Hebdomadaire.16-17:147.","journal-title":"Bull. Epidemiol. Hebdomadaire."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46331-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/502332"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01308-08"},{"key":"e_1_3_2_7_2","unstructured":"Pertussis surveillance in French hospitals: results from a 10 year period. 2007"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002437"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-693X(01)80185-8"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000148936.81366.62"},{"key":"e_1_3_2_11_2","first-page":"351","volume":"16","year":"2008","unstructured":"Guiso, N. 2008. Coqueluche nosocomiale: actualit\u00e9s, m\u00e9thodes diagnostiques et pr\u00e9vention. Hygi\u00e8nes16:351-355.","journal-title":"Hygi\u00e8nes"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-50-5-436"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.467-467.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.6.2330-2333.2000"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.30-35.2005"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1227"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00386-08"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01295-06"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.5.1963-1966.2001"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-007-0403-4"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.10.4925-4929.2005"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4121-4126.2003"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.11.072"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1086\/502541"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00452-8"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00817-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00817-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:28:42Z","timestamp":1646468922000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00817-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["10.1128\/JCM.00817-09"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00817-09","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,10]]},"assertion":[{"value":"2009-04-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-08-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}