{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T14:41:53Z","timestamp":1776696113215,"version":"3.51.2"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The emergence of metallo-\u03b2-lactamase (MBL)-producing isolates is a challenge to routine microbiology laboratories, since there are no standardized methods for detecting such isolates. The aim of this study was to evaluate the accuracy of different phenotypic methods to detect MBL production among\n            <jats:italic>Pseudomonas<\/jats:italic>\n            spp.,\n            <jats:italic>Acinetobacter<\/jats:italic>\n            spp., and enterobacterial isolates, including GIM, IMP, SIM, SPM, and VIM variants. A total of 46 genetically unrelated\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            ,\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            ,\n            <jats:italic>Acinetobacter<\/jats:italic>\n            sp., and enterobacterial strains producing distinct MBLs were tested. Nineteen strains were included as negative controls. The inhibition of bacterial growth and \u03b2-lactam hydrolysis caused by MBL inhibitors (IMBL) also were evaluated. The isolates were tested for MBL production by both a double-disk synergy test (DDST) and a combined disk assay (CD) using imipenem and ceftazidime as substrates in combination with distinct IMBL. One hundred percent sensitivity and specificity were achieved by DDST using 2-mercaptopropionic acid in combination with ceftazidime and imipenem for the detection of MBL production among\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            and\n            <jats:italic>Acinetobacter<\/jats:italic>\n            species isolates, respectively. The CD test showed the same results for detecting MBL-producing enterobacteria by combining imipenem and EDTA, with a 5.0-mm-breakpoint increase in the size of the inhibition zone. Our results indicate that both phenotypic methods to detect MBL-producing isolates should be based on the genera to be tested, regardless of the enzyme produced by such isolates, as well as on the local prevalence of MBL producers.\n          <\/jats:p>","DOI":"10.1128\/jcm.00818-07","type":"journal-article","created":{"date-parts":[[2008,3,6]],"date-time":"2008-03-06T02:09:46Z","timestamp":1204769386000},"page":"2028-2037","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":103,"title":["Metallo-\u03b2-Lactamase Detection: Comparative Evaluation of Double-Disk Synergy versus Combined Disk Tests for IMP-, GIM-, SIM-, SPM-, or VIM-Producing Isolates"],"prefix":"10.1128","volume":"46","author":[{"given":"Renata C.","family":"Pica\u0303o","sequence":"first","affiliation":[{"name":"Laborato\u0301rio ALERTA, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraya S.","family":"Andrade","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio Especial de Microbiologia Cli\u0301nica, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana Gianinni","family":"Nicoletti","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio ALERTA, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eloiza H.","family":"Campana","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio ALERTA, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela C.","family":"Moraes","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio ALERTA, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo E.","family":"Mendes","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio ALERTA, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana C.","family":"Gales","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio ALERTA, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"},{"name":"Laborato\u0301rio Especial de Microbiologia Cli\u0301nica, Universidade Federal de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02467-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.40-43.2000"},{"key":"e_1_3_2_4_2","first-page":"64","year":"2005","unstructured":"Castanheira, M., R. N. Jones, H. S. Sader, R. C. Picao, and A. C. Gales. 2005. Inter-species dissemination of an integron carrying blaVIM-1 between Pseudomonas aeruginosa and Enterobacter cloacae, abstr. C1-84, p. 64. Abstr. 45th Intersci. Conf. Antimicrob. Agents Chemother. American Society for Microbiology, Washington, DC.","journal-title":"Inter-species dissemination of an integron carrying blaVIM-1 between Pseudomonas aeruginosa and Enterobacter cloacae"},{"key":"e_1_3_2_5_2","first-page":"72","year":"2006","unstructured":"Castanheira, M., R. E. Mendes, R. C. Picao, F. P. Pinto, A. M. O. Machado, T. R. Walsh, and A. Gales. 2006. Genetic analysis of a multidrug-resistant (MDR) Enterobacter cloacae producing IMP-1 metallo-\u03b2-lactamase (MBL), abstr. C1-63, p. 72. Abstr. 46th Intersci. Conf. Antimicrob. Agents Chemother. American Society for Microbiology, Washington, DC.","journal-title":"Genetic analysis of a multidrug-resistant (MDR) Enterobacter cloacae producing IMP-1 metallo-\u03b2-lactamase (MBL)"},{"key":"e_1_3_2_6_2","unstructured":"Discrepancy in the metallo-beta-lactamase phenothypic tests results associated with diversity in the promoter region of class 1 integrons 2006"},{"key":"e_1_3_2_7_2","unstructured":"Metallo-beta-lactamase gene blaSPM-1 evaluation of its vicinities in unrelated Pseudomonas aeruginosa strains isolated from distinct Brazilian hospitals 2005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4654-4661.2004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2005.07.004"},{"key":"e_1_3_2_10_2","unstructured":"Performance standards for antimicrobial susceptibility testing 2006"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.10.008"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00879-06"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(00)00222-4"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2004.04.007"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.05.017"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2001.00204.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4623-4629.2003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.2.942-944.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.11.4485-4491.2005"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.516-519.2005"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2753.2005.00598.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5648-5652.2005"},{"key":"e_1_3_2_23_2","first-page":"70","year":"2006","unstructured":"Mendes, R. E., M. Castanheira, M. Toleman, A. Gales, R. C. Picao, and A. C Pignatari. 2006. Characterization of blaIMP-1 dissemination among non-clonally related Acinetobacter spp. (ACB) causing nosocomial infections in a Brazilian teaching hospital in S\u00e3o Paulo, Brazil, abstr. C1-55, p. 70. Abstr. 46th Intersci. Conf. Antimicrob. Agents Chemother. American Society for Microbiology, Washington, DC.","journal-title":"Characterization of blaIMP-1 dissemination among non-clonally related Acinetobacter spp. (ACB) causing nosocomial infections in a Brazilian teaching hospital in S\u00e3o Paulo, Brazil, abstr. C1-55"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01728-06"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4693-4702.2004"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4388-4390.2002"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2006.12.39"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2004.08.013"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi027072i"},{"key":"e_1_3_2_30_2","first-page":"86","year":"2006","unstructured":"Takano, A. P., M. Castanheira, R. E. Mendes, A. P. Penteado, and A. Gales. 2006. Contemporary assessment on prevalence of metallo-\u03b2-lactamase (MBL)-encoding genes in a Brazilian tertiary care university hospital, abstr. C2-100, p. 86. Abstr. 45th Intersci. Conf. Antimicrob. Agents Chemother. American Society for Microbiology, Washington, DC.","journal-title":"Contemporary assessment on prevalence of metallo-\u03b2-lactamase (MBL)-encoding genes in a Brazilian tertiary care university hospital, abstr. C2-100"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1086\/504356"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh512"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.329-332.2004"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf210"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2755-2759.2002"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.306-325.2005"},{"key":"e_1_3_2_37_2","first-page":"110","year":"2006","unstructured":"Xavier, D. E., A. C. Gales, R. E. Mendes, A. C. Pignatari, L. F. Cirilo, L. Santos Filho, and M. Castanheira. 2006. IMP-18-producing Pseudomonas aeruginosa (PSA): increasing diversity of mobile metallo-\u03b2-lactamases (MBL) in Brazil, abstr. C2-420, p. 110. Program Abstr. 46th Intersci. Conf. Antimicrob. Agents Chemother. American Society for Microbiology, Washington, DC.","journal-title":"IMP-18-producing Pseudomonas aeruginosa (PSA): increasing diversity of mobile metallo-\u03b2-lactamases (MBL) in Brazil, abstr. C2-420"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2004.01.002"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3798-3801.2002"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl239"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki390"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00818-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00818-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:23:54Z","timestamp":1646461434000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00818-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["10.1128\/JCM.00818-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00818-07","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,6]]},"assertion":[{"value":"2007-04-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-02-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}