{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:56:08Z","timestamp":1791039368719,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genetic background and the presence of several virulence factors of\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            isolates from intensive care unit (ICU) patients from 14 hospitals in The Netherlands isolated from 1996 until 2006 were investigated. In total, 936 methicillin-susceptible\n            <jats:italic>S. aureus<\/jats:italic>\n            (MSSA) and 7 methicillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            (MRSA) isolates were collected. The genetic background was determined by\n            <jats:italic>spa<\/jats:italic>\n            typing and multilocus sequence typing (MLST). The virulence determinants Panton-Valentine leukocidin (PVL), toxic shock syndrome toxin 1 (TSST-1), and collagen adhesion (CNA) were detected with real-time PCR assays. On the MRSA isolates, mobile resistance staphylococcal cassette chromosome\n            <jats:italic>mec<\/jats:italic>\n            (SCC\n            <jats:italic>mec<\/jats:italic>\n            ) typing was performed. Among the MSSA isolates, 313 different\n            <jats:italic>spa<\/jats:italic>\n            types were observed. A genetic background common to MRSA clones, e.g., MLST clonal complex 1 (CC1), CC5, CC8, CC22, CC30, and CC45, was observed among 62% of the isolates. The remaining isolates were associated with MSSA-related MLST CCs. MLST CC1, CC25, and CC30 were continuously present, and other MLST CCs fluctuated over time. Two percent of the MSSA isolates harbored PVL, 21% had TSST-1, and 46% were positive for CNA. There were no changes in the prevalence of the virulence factors over time. Four MRSA isolates were typed as ST8-MRSA-IV (where ST is the MLST sequence type and IV is the SCC\n            <jats:italic>mec<\/jats:italic>\n            type), two were ST5-MRSA-II, and one was ST228-MRSA-I. All MRSA isolates were PVL, CNA, and TSST-1 negative except for the two ST5-MRSA-II isolates, which were TSST-1 positive. No changes in the\n            <jats:italic>S. aureus<\/jats:italic>\n            genetic background and the prevalence of the virulence factors PVL, CNA, and TSST-1 were observed in ICU patients in The Netherlands over time.\n          <\/jats:p>","DOI":"10.1128\/jcm.00820-09","type":"journal-article","created":{"date-parts":[[2009,10,8]],"date-time":"2009-10-08T01:56:50Z","timestamp":1254967010000},"page":"4090-4095","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Population Structure of\n            <i>Staphylococcus aureus<\/i>\n            Strains Isolated from Intensive Care Unit Patients in The Netherlands over an 11-Year Period (1996 to 2006)"],"prefix":"10.1128","volume":"47","author":[{"given":"M. I. 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