{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T13:09:36Z","timestamp":1791551376256,"version":"4.3.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Brucellosis is a serious problem in Sicily.\n            <jats:italic>Brucella melitensis<\/jats:italic>\n            was identified as the species most frequently isolated in humans in Italy. No data, however, are available about the molecular epidemiological characterization of\n            <jats:italic>Brucella<\/jats:italic>\n            isolates from humans. We have conducted this study to molecularly characterize clinical isolates of\n            <jats:italic>Brucella<\/jats:italic>\n            spp. and to evaluate their antimicrobial susceptibilities. Twenty\n            <jats:italic>Brucella<\/jats:italic>\n            isolates were studied. Differential growth characteristics and DNA polymorphisms such as the restriction patterns of the PCR-amplified\n            <jats:italic>omp2a<\/jats:italic>\n            and\n            <jats:italic>omp2b<\/jats:italic>\n            genes,\n            <jats:italic>rpoB<\/jats:italic>\n            nucleotide sequencing, and multiple-locus variable-number tandem repeat analysis of 16 loci (MLVA-16) were used to characterize the strains. In vitro antibiotic susceptibility was determined by the E-test method on two different agar media, and the results were compared. All isolates were identified as\n            <jats:italic>B. melitensis<\/jats:italic>\n            biovar 3.\n            <jats:italic>rpoB<\/jats:italic>\n            nucleotide sequence analysis allowed the identification of two different genotypes of\n            <jats:italic>B. melitensis<\/jats:italic>\n            biovar 3. On the other hand, the MLVA-16 typing assay recognized 17 distinct genotypes. All isolates were sensitive to all tested antibiotics (rifampin, doxycycline, ciprofloxacin, ceftriaxone, and trimethoprim-sulfamethoxazole), and the Mueller-Hinton agar plate is recommended for antibiotic susceptibility testing by the E-test method. Our findings identify\n            <jats:italic>B. melitensis<\/jats:italic>\n            biovar 3 as the etiological agent isolated in Sicily and encourage the use of both molecular methods, and in particular of the MLVA-16 assay, in epidemiological trace-back analysis. This study represents the first epidemiological data from molecular typing of\n            <jats:italic>Brucella<\/jats:italic>\n            strains circulating in Italy and, in particular, in eastern Sicily.\n          <\/jats:p>","DOI":"10.1128\/jcm.00822-07","type":"journal-article","created":{"date-parts":[[2007,7,19]],"date-time":"2007-07-19T00:54:45Z","timestamp":1184806485000},"page":"2923-2928","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":71,"title":["Molecular Epidemiological and Antibiotic Susceptibility Characterization of\n            <i>Brucella<\/i>\n            Isolates from Humans in Sicily, Italy"],"prefix":"10.1128","volume":"45","author":[{"given":"Cinzia","family":"Marianelli","sequence":"first","affiliation":[{"name":"Dipartimento di Sanita\u0300 Alimentare ed Animale, Istituto Superiore di Sanita\u0300, Viale Regina Elena 299, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Graziani","sequence":"additional","affiliation":[{"name":"Dipartimento di Sanita\u0300 Alimentare ed Animale, Istituto Superiore di Sanita\u0300, Viale Regina Elena 299, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmela","family":"Santangelo","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Vittorio Emanuele, Dipartimento di Microbiologia, Via Plebiscito 628, 951254 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Xibilia","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Vittorio Emanuele, Dipartimento di Microbiologia, Via Plebiscito 628, 951254 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alida","family":"Imbriani","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Vittorio Emanuele, Dipartimento di Microbiologia, Via Plebiscito 628, 951254 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Amato","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Gravina, Dipartimento di Microbiologia, Via Portosalvo 1, 95041 Caltagirone, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Neri","sequence":"additional","affiliation":[{"name":"Azienda Ospedaliera Gravina, Dipartimento di Microbiologia, Via Portosalvo 1, 95041 Caltagirone, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Cuccia","sequence":"additional","affiliation":[{"name":"Azienda Unita\u0300 Sanitaria Locale No. 3, Dipartimento di Prevenzione, Via Santa Maria La Grande 5, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastiano","family":"Rinnone","sequence":"additional","affiliation":[{"name":"Azienda Unita\u0300 Sanitaria Locale No. 3, Dipartimento di Prevenzione, Via Santa Maria La Grande 5, 95124 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Di Marco","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia, Via S. 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