{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:10:27Z","timestamp":1791220227679,"version":"4.1.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rapid identification of pathogens from blood cultures can decrease lengths of stay and improve patient outcomes. We evaluated the accuracy of the Verigene Gram-positive blood culture (BC-GP) nucleic acid test for investigational use only (Nanosphere, Inc., Northbrook, IL) for the identification of Gram-positive bacteria from blood cultures. The detection of resistance genes (\n            <jats:italic>mecA<\/jats:italic>\n            in\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Staphylococcus epidermidis<\/jats:named-content>\n            and\n            <jats:italic>vanA<\/jats:italic>\n            or\n            <jats:italic>vanB<\/jats:italic>\n            in\n            <jats:named-content content-type=\"genus-species\">Enterococcus faecium<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Enterococcus faecalis<\/jats:named-content>\n            ) by the BC-GP assay also was assessed. A total of 186 positive blood cultures (in BacT\/Alert FA bottles) with Gram-positive cocci observed with Gram staining were analyzed using the BC-GP assay. The BC-GP results were compared with the identification and susceptibility profiles obtained with routine methods in the clinical laboratory. Discordant results were arbitrated with additional biochemical, cefoxitin disk, and repeat BC-GP testing. The initial BC-GP organism identification was concordant with routine method results for 94.6% of the blood cultures. Only 40% of the\n            <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n            identifications were correct. The detection of the\n            <jats:italic>mecA<\/jats:italic>\n            gene for 69 blood cultures with only\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            or\n            <jats:named-content content-type=\"genus-species\">S. epidermidis<\/jats:named-content>\n            was concordant with susceptibility testing results. For 3 of 6 cultures with multiple\n            <jats:named-content content-type=\"genus-species\">Staphylococcus<\/jats:named-content>\n            spp.,\n            <jats:italic>mecA<\/jats:italic>\n            detection was reported but was correlated with oxacillin resistance in a species other than\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            or\n            <jats:named-content content-type=\"genus-species\">S. epidermidis<\/jats:named-content>\n            . The detection of\n            <jats:italic>vanA<\/jats:italic>\n            agreed with susceptibility testing results for 45 of 46 cultures with\n            <jats:named-content content-type=\"genus-species\">E. faecalis<\/jats:named-content>\n            or\n            <jats:named-content content-type=\"genus-species\">E. faecium<\/jats:named-content>\n            . Comparison of the mean times to results for each organism group showed that BC-GP results were available 31 to 42 h earlier than phenotypic identifications and 41 to 50 h earlier than susceptibility results.\n          <\/jats:p>","DOI":"10.1128\/jcm.00831-13","type":"journal-article","created":{"date-parts":[[2013,4,18]],"date-time":"2013-04-18T02:47:21Z","timestamp":1366253241000},"page":"2072-2076","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":117,"title":["Evaluation of the Verigene Gram-Positive Blood Culture Nucleic Acid Test for Rapid Detection of Bacteria and Resistance Determinants"],"prefix":"10.1128","volume":"51","author":[{"given":"Christina M.","family":"Wojewoda","sequence":"first","affiliation":[{"name":"Department of Pathology, Fletcher Allen Health Care\/University of Vermont, Burlington, Vermont, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Sercia","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Navas","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Tuohy","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geraldine S.","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary W.","family":"Procop","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra S.","family":"Richter","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2010.02.001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199901213400307"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200107000-00002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000217961.75225.E9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022139"},{"key":"e_1_3_2_7_2","volume-title":"Verigene Gram-positive blood culture nucleic acid test (BC-GP) package insert","author":"Nanosphere, Inc","year":"2012","unstructured":"Nanosphere, Inc. 2012. Verigene Gram-positive blood culture nucleic acid test (BC-GP) package insert. Nanosphere, Inc., Northbrook, IL."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02982-12"},{"key":"e_1_3_2_9_2","volume-title":"CLSI document M35-A2","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. Abbreviated identification of bacteria and yeast; approved guideline\u2014second edition. CLSI document M35-A2. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_10_2","volume-title":"CLSI document M100-S22","author":"Clinical and Laboratory Standards Institute","year":"2012","unstructured":"Clinical and Laboratory Standards Institute. 2012. Performance standards for antimicrobial susceptibility testing; 22nd informational supplement. CLSI document M100-S22. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.2147\/TCRM.S2838"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/656623"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02150-09"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-2-406"},{"key":"e_1_3_2_15_2","volume-title":"Abstr. 112th Gen. Meet. Am. Soc. Microbiol., poster 1163","author":"Anderson C","year":"2012","unstructured":"AndersonC KaulK VossB ThomsonRB. 2012. Abstr. 112th Gen. Meet. Am. Soc. Microbiol., poster 1163."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-013-0021"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00831-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00831-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:35:00Z","timestamp":1646480100000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00831-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7]]},"references-count":15,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1128\/JCM.00831-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00831-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7]]},"assertion":[{"value":"2013-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}