{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:53:01Z","timestamp":1778665981352,"version":"3.51.4"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000106","name":"Saskatchewan Health Research Foundation","doi-asserted-by":"publisher","award":["3380"],"award-info":[{"award-number":["3380"]}],"id":[{"id":"10.13039\/501100000106","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004828","name":"Grand Challenges Canada","doi-asserted-by":"publisher","award":["S5-0398-01"],"award-info":[{"award-number":["S5-0398-01"]}],"id":[{"id":"10.13039\/501100004828","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008920","name":"University of Saskatchewan","doi-asserted-by":"publisher","award":["417069"],"award-info":[{"award-number":["417069"]}],"id":[{"id":"10.13039\/100008920","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A real-time PCR (RT-PCR) assay was designed for the simultaneous identification of\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            and its ciprofloxacin susceptibility status. A SYBR green-based multiplex RT-PCR format was used; it comprised two different forward primers and a common reverse primer to detect single nucleotide polymorphisms (SNPs) in\n            <jats:italic>gyrA<\/jats:italic>\n            of\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            . The primer pairs were evaluated for their sensitivity and specificity using genomic DNA from 254\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            isolates (82 were ciprofloxacin susceptible and 172 were ciprofloxacin resistant) and 23 non-\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            species isolates. The performance of the primers was validated using genomic DNA from 100 different\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            isolates (46 were ciprofloxacin susceptible and 54 were ciprofloxacin resistant) and 52 non-\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            isolates. The latter panel was revalidated by testing 99 (46 isolates were ciprofloxacin susceptible and 53 isolates were ciprofloxacin resistant) of the\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            isolates and 23 non-\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            isolates. These primers detected\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            and its ciprofloxacin susceptibility status with over 99% sensitivity and specificity for all panels tested. This assay has the potential to be an inexpensive and rapid test for the simultaneous identification of\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            and its ciprofloxacin susceptibility status.\n          <\/jats:p>","DOI":"10.1128\/jcm.00855-17","type":"journal-article","created":{"date-parts":[[2017,8,17]],"date-time":"2017-08-17T00:30:27Z","timestamp":1502929827000},"page":"3201-3209","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Multiplex Real-Time PCR Assay for Simultaneous Identification of Neisseria gonorrhoeae and Its Ciprofloxacin Susceptibility Status"],"prefix":"10.1128","volume":"55","author":[{"given":"Sumudu R.","family":"Perera","sequence":"first","affiliation":[{"name":"Vaccine and Infectious Disease Organization\u2014International Vaccine Centre, University of Saskatchewan, Saskatoon, SK, Canada"},{"name":"Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nurul H.","family":"Khan","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization\u2014International Vaccine Centre, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Martin","sequence":"additional","affiliation":[{"name":"National Microbiology Laboratory, Streptococcus and STI Unit, Public Health Agency of Canada, Winnipeg, MB, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Taheri","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization\u2014International Vaccine Centre, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajinder P.","family":"Parti","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization\u2014International Vaccine Centre, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul N.","family":"Levett","sequence":"additional","affiliation":[{"name":"Government of Saskatchewan, Saskatchewan Disease Control Laboratory, Regina, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg B.","family":"Horsman","sequence":"additional","affiliation":[{"name":"Government of Saskatchewan, Saskatchewan Disease Control Laboratory, Regina, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Kusalik","sequence":"additional","affiliation":[{"name":"Department of Computer Science, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo-Anne R.","family":"Dillon","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization\u2014International Vaccine Centre, University of Saskatchewan, Saskatoon, SK, Canada"},{"name":"Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0143304"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJWH.S13427"},{"key":"e_1_3_2_4_2","first-page":"1","article-title":"Antibiotic resistance in Neisseria gonorrhoeae: will infections be untreatable in the future?","volume":"35","author":"Dillon JR","year":"2015","unstructured":"Dillon JR Parti RP Thakur SD . 2015. 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