{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:50:05Z","timestamp":1792734605186,"version":"4.5.0"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Enterobacterial strains producing clavulanic-acid-inhibited extended-spectrum \u03b2-lactamases (ESBLs) are increasingly reported worldwide. Conventional detection of ESBL production remains time-consuming (24 to 48 h). Therefore, the ESBL NDP (Nordmann\/Dortet\/Poirel) test was developed for a rapid identification of ESBLs in\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            . This biochemical test was based on the\n            <jats:italic>in vitro<\/jats:italic>\n            detection of a cephalosporin (cefotaxime) hydrolysis that is inhibited by tazobactam addition. The ESBL activity was evidenced by a color change (red to yellow) of a pH indicator (red phenol) due to carboxyl-acid formation resulting from cefotaxime hydrolysis that was reversed by addition of tazobactam (positive test). The ESBL NDP test was applied to cultured strains (215 ESBL producers and 40 ESBL nonproducers). Its sensitivity and specificity were 92.6% and 100%, respectively. Its sensitivity (100%) was excellent for detection of CTX-M producers. A few ESBL producers (\n            <jats:italic>n<\/jats:italic>\n            = 16) that remained susceptible to cefotaxime were not detected. The test was also evaluated on spiked blood cultures and showed excellent sensitivity and specificity (100% for both). The test was rapid (less than 1 h) and cost-effective. It can be implemented in any health care facility and is well adapted for infection control purposes in particular.\n          <\/jats:p>","DOI":"10.1128\/jcm.00859-12","type":"journal-article","created":{"date-parts":[[2012,7,4]],"date-time":"2012-07-04T22:38:01Z","timestamp":1341441481000},"page":"3016-3022","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":116,"title":["Rapid Detection of Extended-Spectrum-\u03b2-Lactamase-Producing Enterobacteriaceae"],"prefix":"10.1128","volume":"50","author":[{"given":"Patrice","family":"Nordmann","sequence":"first","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Sud, K.-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Sud, K.-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Service de Bact\u00e9riologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, H\u00f4pital de Bic\u00eatre, Assistance Publique\/H\u00f4pitaux de Paris, Facult\u00e9 de M\u00e9decine Paris Sud, K.-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01009-09"},{"key":"e_1_3_2_3_2","unstructured":"Clinical and Laboratory Standards Institute. 2012. Performance standards for antimicrobial susceptibility testing; 21st informational supplement M100\u2013S22 (updated January 2012). CLSI Wayne PA."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","unstructured":"CoqueTM\nBaqueroF\nCantonR. 2008. Increasing prevalence of ESBL-producing Enterobacteriaceae in Europe. Euro Surveill. 13:pii=19044. http:\/\/www.eurosurveillance.org\/ViewArticle.aspx?ArticleId=19044.","DOI":"10.2807\/ese.13.47.19044-en"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks156"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01846.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06852-11"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl483"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e3283398dc1"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.02.008"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47625-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.4.1257-1262.2006"},{"key":"e_1_3_2_14_2","first-page":"172","article-title":"Addressing the challenge of extended-spectrum \u03b2-lactamases","volume":"10","author":"Zahar JR","year":"2009","unstructured":"ZaharJR. 2009. Addressing the challenge of extended-spectrum \u03b2-lactamases. Curr. Opin. Investig. Drugs 10:172\u2013180.","journal-title":"Curr. Opin. Investig. Drugs"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00859-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.00859-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:13:39Z","timestamp":1646478819000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.00859-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9]]},"references-count":13,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1128\/JCM.00859-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.00859-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9]]},"assertion":[{"value":"2012-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-08-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}