{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:03:41Z","timestamp":1790517821827,"version":"4.1.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Black-grain mycetomas are subcutaneous devastating chronic infections due to several dematiaceous fungi. They are diagnosed mostly in tropical countries. Identification of these fungi with standard mycological procedures is difficult because of their poor or delayed sporulation. The aim of this study was thus to assess the accuracy of molecular identification of these fungi. A total of 54 strains, mostly of clinical origin, were used, including 15<jats:italic>Madurella mycetomatis<\/jats:italic>, 6<jats:italic>Madurella grisea<\/jats:italic>, 12<jats:italic>Leptosphaeria senegalensis<\/jats:italic>, 4<jats:italic>Leptosphaeria tompkinsii<\/jats:italic>, 6<jats:italic>Pyrenochaeta<\/jats:italic>spp., 4<jats:italic>Curvularia lunata<\/jats:italic>, and 7<jats:italic>Exophiala jeanselmei<\/jats:italic>strains. The internal transcribed spacer 1 (ITS1)-5.8S-ITS2 DNA region was amplified by using universal fungal primers and sequenced. Both intra- and interspecies sequence similarities were assessed.<jats:italic>Madurella mycetomatis<\/jats:italic>appeared to be a homogeneous species. More intraspecies variations were found for<jats:italic>C. lunata<\/jats:italic>and<jats:italic>E. jeanselmei<\/jats:italic>, leading, in some instances, to changes in the initial identification.<jats:italic>L. senegalensis<\/jats:italic>and<jats:italic>L. tompkinsii<\/jats:italic>showed intraspecies similarities of &gt;99%, but similarity between the two species was &lt;88%. Intergenera and interspecies variations were important, with sequence homologies of &lt;81% between genera. In contrast,<jats:italic>Pyrenochaeta romeroi<\/jats:italic>and<jats:italic>M. grisea<\/jats:italic>appeared to be heterogeneous, with intraspecies similarities of 40 to 100% and 53 to 100%, respectively, which suggest either erroneous identification or the need for taxonomic revision. Epidemiological and therapeutic studies could benefit from a precise identification of the fungi responsible for black-grain mycetoma based not only on phenotypical characteristics but also on ITS sequencing.<\/jats:p>","DOI":"10.1128\/jcm.00862-06","type":"journal-article","created":{"date-parts":[[2006,10,4]],"date-time":"2006-10-04T17:59:53Z","timestamp":1159984793000},"page":"3517-3523","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":86,"title":["Molecular Identification of Black-Grain Mycetoma Agents"],"prefix":"10.1128","volume":"44","author":[{"given":"Marie","family":"Desnos-Ollivier","sequence":"first","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS FRE2849, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ste\u0301phane","family":"Bretagne","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS FRE2849, Institut Pasteur, Paris"},{"name":"Laboratoire de Parasitologie-Mycologie, AP-HP, Ho\u0302pital Henri Mondor, Cre\u0301teil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Dromer","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS FRE2849, Institut Pasteur, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Lortholary","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS FRE2849, Institut Pasteur, Paris"},{"name":"Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine, AP-HP, Ho\u0302pital Necker-Enfants-Malades, Service des Maladies Infectieuses et Tropicales, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Dannaoui","sequence":"additional","affiliation":[{"name":"Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, Unite\u0301 de Mycologie Mole\u0301culaire, CNRS FRE2849, Institut Pasteur, Paris"},{"name":"Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine, AP-HP, Ho\u0302pital Europe\u0301en Georges Pompidou, Unite\u0301 de Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4537-4541.2003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.12.5813-5816.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.10.3175-3178.1999"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01131-4"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2004.00964.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1111\/j.1439-0507.1993.tb00693.x","volume":"36","year":"1993","unstructured":"de Hoog, G. 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