{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:17:33Z","timestamp":1791299853061,"version":"4.1.0"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001702","name":"AO Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001702","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001702","name":"AO Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001702","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001702","name":"AO Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001702","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Staphylococcus epidermidis<\/jats:named-content>\n            has emerged as an important opportunistic pathogen causing orthopedic-device-related infections (ODRI). This study investigated the association of genome variation and phenotypic features of the infecting\n            <jats:named-content content-type=\"genus-species\">S. epidermidis<\/jats:named-content>\n            isolate with the clinical outcome for the infected patient.\n            <jats:named-content content-type=\"genus-species\">S. epidermidis<\/jats:named-content>\n            isolates were collected from 104 patients with ODRI. Their clinical outcomes were evaluated, after an average of 26 months, as either \u201ccured\u201d or \u201cnot cured.\u201d The isolates were tested for antibiotic susceptibility and biofilm formation. Whole-genome sequencing was performed on all isolates, and genomic variation was related to features associated with \u201ccured\u201d and \u201cnot cured.\u201d Strong biofilm formation and aminoglycoside resistance were associated with a \u201cnot-cured\u201d outcome (\n            <jats:italic>P<\/jats:italic>\n            = 0.031 and\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001, respectively). Based on gene-by-gene analysis, some accessory genes were more prevalent in isolates from the \u201cnot-cured\u201d group. These included the biofilm-associated\n            <jats:italic>bhp<\/jats:italic>\n            gene, the antiseptic resistance\n            <jats:italic>qacA<\/jats:italic>\n            gene, the cassette chromosome recombinase-encoding genes\n            <jats:italic>ccrA<\/jats:italic>\n            and\n            <jats:italic>ccrB<\/jats:italic>\n            , and the IS\n            <jats:italic>256<\/jats:italic>\n            -like transposase gene. This study identifies biofilm formation and antibiotic resistance as associated with poor outcome in\n            <jats:named-content content-type=\"genus-species\">S. epidermidis<\/jats:named-content>\n            ODRI. Whole-genome sequencing identified specific genes associated with a \u201cnot-cured\u201d outcome that should be validated in future studies. (The study has been registered at ClinicalTrials.gov with identifier NCT02640937.)\n          <\/jats:p>","DOI":"10.1128\/jcm.00881-17","type":"journal-article","created":{"date-parts":[[2017,8,10]],"date-time":"2017-08-10T00:40:15Z","timestamp":1502325615000},"page":"3089-3103","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome"],"prefix":"10.1128","volume":"55","author":[{"given":"Virginia","family":"Post","sequence":"first","affiliation":[{"name":"AO Research Institute Davos, Davos, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Llinos G.","family":"Harris","sequence":"additional","affiliation":[{"name":"Microbiology and Infectious Diseases, Institute of Life Science, Swansea University Medical School, Swansea, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Morgenstern","sequence":"additional","affiliation":[{"name":"Department of Orthopedic and Trauma Surgery, University Hospital Basel, Basel, Switzerland"},{"name":"Department of Trauma Surgery, Trauma Centre Murnau, Murnau, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardos","family":"Mageiros","sequence":"additional","affiliation":[{"name":"Microbiology and Infectious Diseases, Institute of Life Science, Swansea University Medical School, Swansea, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D.","family":"Hitchings","sequence":"additional","affiliation":[{"name":"Microbiology and Infectious Diseases, Institute of Life Science, Swansea University Medical School, Swansea, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume","family":"M\u00e9ric","sequence":"additional","affiliation":[{"name":"The Milner Centre for Evolution, Department of Biology &amp; Biochemistry, University of Bath, Bath, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Pascoe","sequence":"additional","affiliation":[{"name":"The Milner Centre for Evolution, Department of Biology &amp; Biochemistry, University of Bath, Bath, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel K.","family":"Sheppard","sequence":"additional","affiliation":[{"name":"The Milner Centre for Evolution, Department of Biology &amp; Biochemistry, University of Bath, Bath, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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